In vitro study on the pulmonary cytotoxicity of amiodarone

Mohammed T. Alsamri, Thachillath Pramathan, Abdul Kader Souid

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Context: Amiodarone (an iodinated benzofuran) is a Class III antiarrhythmic drug that produces significant pulmonary disease. Proposed mechanisms of this cytotoxicity include necrosis, apoptosis, mitochondrial dysfunction and glutathione depletion. Objective: This study was designed primarily to explore whether amiodarone impairs lung tissue cellular bioenergetics in BALB/c and Taylor Outbred mice. Materials and methods: Cellular respiration (mitochondrial O2 consumption), ATP, caspase activity and glutathione were measured in lung fragments incubated in vitro with 22μM amiodarone for several hours. Results: Without amiodarone, lung tissue cellular mitochondrial O2 consumption decayed exponentially with time, showing two distinct phases sharply separated at t150min. The rate of cellular respiration was 6-10-fold higher in the late phase compared to the early phase (p<0.0001). Lung tissue ATP also decayed exponentially with time, suggesting "uncoupling oxidative phosphorylation" was the responsible mechanism (low cellular ATP with high mitochondrial O2 consumption, resulting in rapid depletion of cellular metabolic fuels). Although intracellular caspase activity increased exponentially with time, the uncoupling was not prevented by the pancaspase inhibitor zVAD-fmk (N-benzyloxycarbonyl-val-ala-asp (O-methyl)-fluoromethylketone). The same profiles were noted in the presence of amiodarone; but cellular ATP decayed 50% faster. Cellular glutathione for untreated tissue was 560±287pmolmg -1 (n=12) and for treated tissue was 490±226pmolmg -1 (n=12, p=0.5106). Conclusion: Uncoupling oxidative phosphorylation was demonstrated in untreated mouse lung tissues. Amiodarone lowered cellular ATP. Further studies are needed to explore the susceptibility of the lung to these deleterious insults and their relevance to human diseases.

Original languageEnglish
Pages (from-to)610-616
Number of pages7
JournalToxicology Mechanisms and Methods
Volume23
Issue number8
DOIs
Publication statusPublished - Oct 2013

Keywords

  • Amiodarone
  • Apoptosis
  • Bioenergetics
  • Caspases
  • Cytotoxicity
  • Glutathione
  • Mitochondria
  • Monobromobimane

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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