Induction of innate immunity by IL-2-expressing Salmonella confers protection against lethal infection

Basel K. Al-Ramadi, Nada Mustafa, Mounir AbouHaidar, Maria J. Fernandez-Cabezudo

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Previously, we demonstrated that an attenuated Salmonella strain expressing IL-2 (designated GIDIL2) is cleared from the circulation at a much faster rate than the non-cytokine-expressing parental strain (designated BRD509). These findings suggested that IL-2 expression led to the rapid induction of innate immune responses that, in turn, accounted for the accelerated rate of bacterial clearance. In the present study, the mechanism by which this early antibacterial response is mediated was investigated. We demonstrate that as early as 2h after infection with GIDIL2, but not BRD509, peritoneal excudate cells exhibited enhanced NK cytotoxic activity and upregulated NOS2 mRNA and NO production. This early response coincided with an enhancement of GIDIL2 clearance from the peritoneal cavity, first evidenced 22h post-infection. Moreover, it conferred a high level of protection against virulent Salmonella challenge given within 16-20h of GIDIL2 administration. These findings highlight the importance of innate immunity in the control of early bacterial proliferation and demonstrate the rapidity by which these responses are induced following bacterial entry.

Original languageEnglish
Pages (from-to)763-770
Number of pages8
JournalMolecular Immunology
Issue number13
Publication statusPublished - Mar 2003


  • Innate immunity
  • Interleukin-2
  • NK cells
  • NO production
  • Salmonella

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology


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