Abstract
Ethanol has been reported to modulate arterial dilation and insulin secretion. ATP-inhibited K+ channels (KATP) are reported to have regulatory roles during these events. In the present study, the effect of ethanol on K+ currents activated by the KATP channel opener cromakalim was investigated in follicular cells of Xenopus oocytes. The results indicate that ethanol in the concentration range of 10-300 mM (approximately 0.06%-2% v/v) reversibly inhibits the cromakalim-induced K+ currents. The 50% of maximal ethanol effect was obtained at 53 mM. Inhibition of cromakalim-activated K+ current by ethanol was non-competitive. In oocytes treated with the Ca2+-chelator BAPTA, inhibition of cromakalim-induced K+ currents by ethanol was not altered, suggesting that Ca2+-activated second messenger pathways were not involved in the actions of ethanol. Similarly, currents activated by 8-Br-cAMP were also inhibited by ethanol, but the ethanol inhibition of 8-Br-cAMP-activated currents was significantly less than inhibition of cromakalim-activated currents by ethanol. These results indicate that cromakalim-activated K+ currents in follicular cells of Xenopus oocytes were modulated by ethanol.
Original language | English |
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Pages (from-to) | 80-85 |
Number of pages | 6 |
Journal | Naunyn-Schmiedeberg's Archives of Pharmacology |
Volume | 367 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1 2003 |
Keywords
- Cromakalim
- Ethanol
- K channels
- Xenopus oocytes
ASJC Scopus subject areas
- Pharmacology