InsP3-induced Ca2+ release in dorsal root ganglion neurones

Natalia Svichar; Anatoly Shmigol; Alexej Verkhratsky; Platon Kostyuk

doi:10.1016/S0304-3940(97)00313-3

InsP₃-induced Ca²⁺ release in dorsal root ganglion neurones

Natalia Svichar, Anatoly Shmigol, Alexej Verkhratsky, Platon Kostyuk

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca²⁺ concentration ([Ca²⁺](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca²⁺](i) elevation. Removal of extracellular Ca²⁺ had no effect on both ATP-induced current and [Ca²⁺](i) transient. The ATP-induced Ca²⁺ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca²⁺ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP₃) production, trigger the InsP₃-induced Ca²⁺ release from intracellular stores.

Original language	English
Pages (from-to)	107-110
Number of pages	4
Journal	Neuroscience Letters
Volume	227
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(97)00313-3
Publication status	Published - May 16 1997
Externally published	Yes

Keywords

Ca release
Caffeine
Cytoplasmic calcium
Inositol-trisphosphate (InsP)
Purinoreceptors
Sensory neurones

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/S0304-3940(97)00313-3

Cite this

@article{c158d6875e8f48c0999cfce520513b39,

title = "InsP3-induced Ca2+ release in dorsal root ganglion neurones",

abstract = "The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca2+ concentration ([Ca2+](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca2+](i) elevation. Removal of extracellular Ca2+ had no effect on both ATP-induced current and [Ca2+](i) transient. The ATP-induced Ca2+ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca2+ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP3) production, trigger the InsP3-induced Ca2+ release from intracellular stores.",

keywords = "Ca release, Caffeine, Cytoplasmic calcium, Inositol-trisphosphate (InsP), Purinoreceptors, Sensory neurones",

author = "Natalia Svichar and Anatoly Shmigol and Alexej Verkhratsky and Platon Kostyuk",

note = "Funding Information: This research was supported in part by INTAS grant to P.K. and by Wellcome Trust Collaborative Research Grant to A.V. and by CRDF grant 927 to A.S. The authors thank Ludmila Grigorovitch for excellent technical assistance.",

year = "1997",

month = may,

day = "16",

doi = "10.1016/S0304-3940(97)00313-3",

language = "English",

volume = "227",

pages = "107--110",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - InsP3-induced Ca2+ release in dorsal root ganglion neurones

AU - Svichar, Natalia

AU - Shmigol, Anatoly

AU - Verkhratsky, Alexej

AU - Kostyuk, Platon

N1 - Funding Information: This research was supported in part by INTAS grant to P.K. and by Wellcome Trust Collaborative Research Grant to A.V. and by CRDF grant 927 to A.S. The authors thank Ludmila Grigorovitch for excellent technical assistance.

PY - 1997/5/16

Y1 - 1997/5/16

N2 - The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca2+ concentration ([Ca2+](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca2+](i) elevation. Removal of extracellular Ca2+ had no effect on both ATP-induced current and [Ca2+](i) transient. The ATP-induced Ca2+ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca2+ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP3) production, trigger the InsP3-induced Ca2+ release from intracellular stores.

AB - The intracellular calcium signaling was studied on subpopulation of freshly isolated adult mouse dorsal root ganglia (DRG) neurones with large somatas (30-45 μ in diameter). The cytoplasmic Ca2+ concentration ([Ca2+](i)) was measured using indo-1 based microfluorimetry. The extracellular application of ATP (100 μM) triggered both inward current and [Ca2+](i) elevation. Removal of extracellular Ca2+ had no effect on both ATP-induced current and [Ca2+](i) transient. The ATP-induced Ca2+ elevation was inhibited by intracellular perfusion of DRG neurones with 20 μM heparin, or by cells incubation with thapsigargin or ryanodine. We conclude that mouse proprioceptive sensory neurones are endowed with Ca2+ - impermeable ionotropic P(2X) purinoreceptors and metabotropic P(2Y) purinoreceptors, which, by means of phospholipase C-drive inositol- trisphosphate (InsP3) production, trigger the InsP3-induced Ca2+ release from intracellular stores.

KW - Ca release

KW - Caffeine

KW - Cytoplasmic calcium

KW - Inositol-trisphosphate (InsP)

KW - Purinoreceptors

KW - Sensory neurones

UR - http://www.scopus.com/inward/record.url?scp=0030612410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030612410&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(97)00313-3

DO - 10.1016/S0304-3940(97)00313-3

M3 - Article

C2 - 9180215

AN - SCOPUS:0030612410

SN - 0304-3940

VL - 227

SP - 107

EP - 110

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2

ER -