Insulin releasing properties of the temporin family of antimicrobial peptides

Yasser H.A. Abdel-Wahab; Lamin Marenah; Peter R. Flatt; J. Michael Conlon

doi:10.2174/092986607781483822

Insulin releasing properties of the temporin family of antimicrobial peptides

Yasser H.A. Abdel-Wahab, Lamin Marenah, Peter R. Flatt, J. Michael Conlon

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Temporin-1Vb, -1Oe, -1DRb, and -1TGb (10^-8 - 10^-6M) produced significant (p<0.05) and concentration-dependent stimulatory effects on insulin secretion from clonal rat BRIN-BD11 cells without increased release of lactate dehydrogenase. Temporin-1Va and temporin-1Vc (10^-8 - 10^-6M) also stimulated insulin-release but were cytotoxic at 10 ^-6M. Temporin-1DRa was without effect. The temporins at 10 ^-7 M had no effect on intracellular calcium concentrations suggesting that they stimulate insulin release via a K_ATP channel- independent pathway.

Original language	English
Pages (from-to)	702-707
Number of pages	6
Journal	Protein and Peptide Letters
Volume	14
Issue number	7
DOIs	https://doi.org/10.2174/092986607781483822
Publication status	Published - Jul 2007
Externally published	Yes

Keywords

Amphibian skin
Cytolysis
Insulin secretion
Temporin

ASJC Scopus subject areas

Structural Biology
Biochemistry

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10.2174/092986607781483822

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title = "Insulin releasing properties of the temporin family of antimicrobial peptides",

abstract = "Temporin-1Vb, -1Oe, -1DRb, and -1TGb (10-8 - 10-6M) produced significant (p<0.05) and concentration-dependent stimulatory effects on insulin secretion from clonal rat BRIN-BD11 cells without increased release of lactate dehydrogenase. Temporin-1Va and temporin-1Vc (10-8 - 10-6M) also stimulated insulin-release but were cytotoxic at 10 -6M. Temporin-1DRa was without effect. The temporins at 10 -7 M had no effect on intracellular calcium concentrations suggesting that they stimulate insulin release via a KATP channel- independent pathway.",

keywords = "Amphibian skin, Cytolysis, Insulin secretion, Temporin",

author = "Abdel-Wahab, {Yasser H.A.} and Lamin Marenah and Flatt, {Peter R.} and Conlon, {J. Michael}",

year = "2007",

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language = "English",

volume = "14",

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journal = "Protein and Peptide Letters",

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T1 - Insulin releasing properties of the temporin family of antimicrobial peptides

AU - Abdel-Wahab, Yasser H.A.

AU - Marenah, Lamin

AU - Flatt, Peter R.

AU - Conlon, J. Michael

PY - 2007/7

Y1 - 2007/7

N2 - Temporin-1Vb, -1Oe, -1DRb, and -1TGb (10-8 - 10-6M) produced significant (p<0.05) and concentration-dependent stimulatory effects on insulin secretion from clonal rat BRIN-BD11 cells without increased release of lactate dehydrogenase. Temporin-1Va and temporin-1Vc (10-8 - 10-6M) also stimulated insulin-release but were cytotoxic at 10 -6M. Temporin-1DRa was without effect. The temporins at 10 -7 M had no effect on intracellular calcium concentrations suggesting that they stimulate insulin release via a KATP channel- independent pathway.

AB - Temporin-1Vb, -1Oe, -1DRb, and -1TGb (10-8 - 10-6M) produced significant (p<0.05) and concentration-dependent stimulatory effects on insulin secretion from clonal rat BRIN-BD11 cells without increased release of lactate dehydrogenase. Temporin-1Va and temporin-1Vc (10-8 - 10-6M) also stimulated insulin-release but were cytotoxic at 10 -6M. Temporin-1DRa was without effect. The temporins at 10 -7 M had no effect on intracellular calcium concentrations suggesting that they stimulate insulin release via a KATP channel- independent pathway.

KW - Amphibian skin

KW - Cytolysis

KW - Insulin secretion

KW - Temporin

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Insulin releasing properties of the temporin family of antimicrobial peptides

Abstract

Keywords

ASJC Scopus subject areas

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