Integrating the porcine physical and linkage map using cosmid‐derived markers

H. Ellegren, B. Chowdhary, M. Johansson, L. Andersson

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


An essential part in the development of informative linkage maps is to include genetic markers that have been anchored by physical mapping. Here a set of 18 porcine cosmid‐derived genetic markers are reported that have been mapped by linkge analysis, and that also have been physically localized by fluorescence in situ hybridization (FISH). Three different strategies were used to establish polymorphic markers from the cosmid clones. Firstly, dinucleotide microsatellite loci were derived by sequencing cosmid subclones containing (CA), repeats. Secondly, variable SINE 3′ poly(A) tracts (SINEVA) were identified by direct SINE‐PCR amplification of cosmid clones. Thirdly, the cosmids were used in Southern blot hybridization to detect restriction fragment length polymorphisms (RFLPs). Compared with the most recent consensus compilation of the porcine gene map, the present assignment of markers to chromosomes Zp, 3, 4, 10, 12q, and 16 represents the first loci mapped to these chromosomes, for which linkage as well as in situ data are now available.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalAnimal Genetics
Issue number3
Publication statusPublished - Jun 1994
Externally publishedYes


  • PCR
  • SINE
  • gene mapping
  • in situ hybridization
  • microsatellites
  • pig

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics


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