Intracellular stores and calcium signalling in mammalian sensory neurones

P. G. Kostyuk; A. V. Shmigol

doi:10.1016/S0302-4598(96)05132-X

Intracellular stores and calcium signalling in mammalian sensory neurones

P. G. Kostyuk, A. V. Shmigol

Research output: Contribution to journal › Conference article › peer-review

10 Citations (Scopus)

Abstract

The:injection of Ca ions into the cytoplasm through coordinated activity of calcium-permeable channels in the surface membrane and the membranes of intracellular organelles is the main source of temporary elevation of the cytoplasmic free Ca²⁺ level ('the calcium signal') which triggers cellular responses to various stimuli. In our experiments the contribution of intracellular Ca²⁺ stores to calcium signal generation was studied in two subpopulations of dorsal root ganglion (DRG) neurones using a combination of patch-clamp technique for measuring transmembrane currents and fluorescent indicator indo-1 for estimating the changes in the cytoplasmic free Ca²⁺ level. Ca-induced Ca²⁺ release (CICR) and inositol(1,4,5)-triphosphate (IP₃)-induced Ca²⁺ release (IICR) were found to be functional only in certain types of sensory neuronal cell, presumably in neurones transmitting tactile and proprioceptive information. Mitochondrial Ca²⁺ accumulation and release was found to participate in the shaping of calcium signals in both subpopulations of DRG neurones.

Original language	English
Pages (from-to)	197-205
Number of pages	9
Journal	Bioelectrochemistry and Bioenergetics
Volume	42
Issue number	2
DOIs	https://doi.org/10.1016/S0302-4598(96)05132-X
Publication status	Published - May 1997
Externally published	Yes
Event	Proceedings of the 1996 13th International Symposium on Bioelectrochemistry and Bioenergetics - Ein Gedi, Isr Duration: Jan 7 1996 → Jan 12 1996

Keywords

Calcium signalling
Sensory neurones

ASJC Scopus subject areas

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

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10.1016/S0302-4598(96)05132-X

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title = "Intracellular stores and calcium signalling in mammalian sensory neurones",

abstract = "The:injection of Ca ions into the cytoplasm through coordinated activity of calcium-permeable channels in the surface membrane and the membranes of intracellular organelles is the main source of temporary elevation of the cytoplasmic free Ca2+ level ('the calcium signal') which triggers cellular responses to various stimuli. In our experiments the contribution of intracellular Ca2+ stores to calcium signal generation was studied in two subpopulations of dorsal root ganglion (DRG) neurones using a combination of patch-clamp technique for measuring transmembrane currents and fluorescent indicator indo-1 for estimating the changes in the cytoplasmic free Ca2+ level. Ca-induced Ca2+ release (CICR) and inositol(1,4,5)-triphosphate (IP3)-induced Ca2+ release (IICR) were found to be functional only in certain types of sensory neuronal cell, presumably in neurones transmitting tactile and proprioceptive information. Mitochondrial Ca2+ accumulation and release was found to participate in the shaping of calcium signals in both subpopulations of DRG neurones.",

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AU - Kostyuk, P. G.

AU - Shmigol, A. V.

PY - 1997/5

Y1 - 1997/5

N2 - The:injection of Ca ions into the cytoplasm through coordinated activity of calcium-permeable channels in the surface membrane and the membranes of intracellular organelles is the main source of temporary elevation of the cytoplasmic free Ca2+ level ('the calcium signal') which triggers cellular responses to various stimuli. In our experiments the contribution of intracellular Ca2+ stores to calcium signal generation was studied in two subpopulations of dorsal root ganglion (DRG) neurones using a combination of patch-clamp technique for measuring transmembrane currents and fluorescent indicator indo-1 for estimating the changes in the cytoplasmic free Ca2+ level. Ca-induced Ca2+ release (CICR) and inositol(1,4,5)-triphosphate (IP3)-induced Ca2+ release (IICR) were found to be functional only in certain types of sensory neuronal cell, presumably in neurones transmitting tactile and proprioceptive information. Mitochondrial Ca2+ accumulation and release was found to participate in the shaping of calcium signals in both subpopulations of DRG neurones.

AB - The:injection of Ca ions into the cytoplasm through coordinated activity of calcium-permeable channels in the surface membrane and the membranes of intracellular organelles is the main source of temporary elevation of the cytoplasmic free Ca2+ level ('the calcium signal') which triggers cellular responses to various stimuli. In our experiments the contribution of intracellular Ca2+ stores to calcium signal generation was studied in two subpopulations of dorsal root ganglion (DRG) neurones using a combination of patch-clamp technique for measuring transmembrane currents and fluorescent indicator indo-1 for estimating the changes in the cytoplasmic free Ca2+ level. Ca-induced Ca2+ release (CICR) and inositol(1,4,5)-triphosphate (IP3)-induced Ca2+ release (IICR) were found to be functional only in certain types of sensory neuronal cell, presumably in neurones transmitting tactile and proprioceptive information. Mitochondrial Ca2+ accumulation and release was found to participate in the shaping of calcium signals in both subpopulations of DRG neurones.

KW - Calcium signalling

KW - Sensory neurones

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VL - 42

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EP - 205

JO - Bioelectrochemistry and Bioenergetics

JF - Bioelectrochemistry and Bioenergetics

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T2 - Proceedings of the 1996 13th International Symposium on Bioelectrochemistry and Bioenergetics

Y2 - 7 January 1996 through 12 January 1996

ER -

Intracellular stores and calcium signalling in mammalian sensory neurones

Abstract

Keywords

ASJC Scopus subject areas

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