TY - JOUR
T1 - Investigating acrylamide mitigation by potential probiotics Bifidobacterium breve and Lactiplantibacillus plantarum
T2 - Optimization, in vitro gastrointestinal conditions, and mechanism
AU - Albedwawi, Amal S.
AU - Al Sakkaf, Reem
AU - Osaili, Tareq M.
AU - Yusuf, Ahmed
AU - Olaimat, Amin
AU - Liu, Shao Quan
AU - Palmisano, Giovanni
AU - Shah, Nagendra P.
AU - Ayyash, Mutamed M.
N1 - Funding Information:
This research and APC were funded by United Arab Emirates University (UAEU) , Al-Ain, UAE.
Funding Information:
This research and APC were funded by United Arab Emirates University (UAEU), Al-Ain, UAE.The authors are very grateful to Dubai Central Laboratory and Khalifa University for their support. The authors are also thankful to the reviewers for their valuable comments.
Publisher Copyright:
© 2022 The Authors
PY - 2022/6/15
Y1 - 2022/6/15
N2 - This paper investigated the ability of lactic acid bacteria (LAB) and Bifidobacterium to remove acrylamide (AA). The mechanism(s) of the AA removal was examined by various microscopic techniques. After screening 40 isolates, Bifidobacterium breve and Lactiplantibacillus plantarum exhibited higher acrylamide (AA) reduction by 35% and 36%, respectively. pH (4.5–6.5), incubation temperature (32–42 °C), incubation time (14–22 h), and NaCl (0–3 g/100 g) were employed using Box-Behnken design (BBD) to investigate the AA removal. As a result, the AA removal ranged from 47.7 to 65.0% for B. breve and 5.8%–8.0% for Lb. plantarum. Under the in vitro gastrointestinal conditions, B. breve removed up to 41.2% of acrylamide while Lb. plantarum reduced around 53.5% of acrylamide. In addition, both strains tolerated low pH levels. TEM images showed that cells of both bacteria increased after culturing with acrylamide. Elements analysis of Lb. plantarum and the most dominant elements are C, N, and O with atomic % of 41.95, 17.63, and 31.66, respectively. FTIR exhibited that C[dbnd]O, C–O, and N–H were main functional groups associated with AA adsorption in Lb. plantarum and B. breve. The zeta potential values of B. breve and Lb. plantarum are −15.47 and −25.87 mV, respectively.
AB - This paper investigated the ability of lactic acid bacteria (LAB) and Bifidobacterium to remove acrylamide (AA). The mechanism(s) of the AA removal was examined by various microscopic techniques. After screening 40 isolates, Bifidobacterium breve and Lactiplantibacillus plantarum exhibited higher acrylamide (AA) reduction by 35% and 36%, respectively. pH (4.5–6.5), incubation temperature (32–42 °C), incubation time (14–22 h), and NaCl (0–3 g/100 g) were employed using Box-Behnken design (BBD) to investigate the AA removal. As a result, the AA removal ranged from 47.7 to 65.0% for B. breve and 5.8%–8.0% for Lb. plantarum. Under the in vitro gastrointestinal conditions, B. breve removed up to 41.2% of acrylamide while Lb. plantarum reduced around 53.5% of acrylamide. In addition, both strains tolerated low pH levels. TEM images showed that cells of both bacteria increased after culturing with acrylamide. Elements analysis of Lb. plantarum and the most dominant elements are C, N, and O with atomic % of 41.95, 17.63, and 31.66, respectively. FTIR exhibited that C[dbnd]O, C–O, and N–H were main functional groups associated with AA adsorption in Lb. plantarum and B. breve. The zeta potential values of B. breve and Lb. plantarum are −15.47 and −25.87 mV, respectively.
KW - INFOGEST
KW - Maillard reaction
KW - Scanning Electron microscopy
KW - Transmission electron microscopy
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U2 - 10.1016/j.lwt.2022.113553
DO - 10.1016/j.lwt.2022.113553
M3 - Article
AN - SCOPUS:85130148167
SN - 0023-6438
VL - 163
JO - LWT - Food Science and Technology
JF - LWT - Food Science and Technology
M1 - 113553
ER -