Irc20 Regulates the Yeast Endogenous 2-μm Plasmid Levels by Controlling Flp1

Deena Jalal, Jisha Chalissery, Ahmed H. Hassan

Research output: Contribution to journalArticlepeer-review


The endogenous yeast 2-μm plasmid while innocuous to the host, needs to be properly regulated to avoid a toxic increase in copy number. The plasmid copy number control system is under the control of the plasmid encoded recombinase, Flp1. In case of a drop in 2-μm plasmid levels due to rare plasmid mis-segregation events, the Flp1 recombinase together with the cell’s homologous recombination machinery, produce multiple copies of the 2-μm plasmid that are spooled during DNA replication. The 2-μm plasmid copy number is tightly regulated by controlled expression of Flp1 as well as its ubiquitin and SUMO modification. Here, we identify a novel regulator of the 2-μm plasmid, the ATPase, ubiquitin ligase, Irc20. Irc20 was initially identified as a homologous recombination regulator, and here we uncover a new role for Irc20 in maintaining the 2-μm plasmid copy number and segregation through regulating Flp1 protein levels in the cell.

Original languageEnglish
Article number221
JournalFrontiers in Molecular Biosciences
Publication statusPublished - Nov 19 2020


  • 2-μm plasmid
  • Flp1
  • Irc20
  • SUMO
  • homologous recombination
  • ubiquitin ligase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)


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