Isolation and characterization of exosomes released by EBV-immortalized cells

Gulfaraz Khan, Waqar Ahmed

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)


Epstein-Barr virus is an oncogenic herpesvirus associated with several human malignancies. Although the details of the molecular steps involved in EBV-mediated cell transformation and immune evasion are not fully known, a number of viral products, including EBV latent proteins and non-protein coding RNAs have been shown to be involved, directly or indirectly in these processes. In recent years, a growing body of data indicates that some viruses are able to transport selected products to neighboring cells and induce biological changes by exploiting the exosome secretory pathway. Exosomes are nanovesicles secreted by virtually all cell types and present in most body fluids. Here, we describe the protocols used in our laboratory to isolate and characterize exosomes from EBV-infected, noninfected, and transfected cell lines.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • CD63
  • Differential ultracentrifugation
  • EBV
  • Electron microscopy
  • Exosomes
  • Flotillin
  • RT-PCR
  • Western blotting

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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