TY - JOUR
T1 - Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultivars
AU - Ji, Sang Hye
AU - Gururani, Mayank Anand
AU - Chun, Se Chul
N1 - Funding Information:
This work was supported by the Konkuk University Research Fund in 2011. The Korean Agricultural Culture Collection (KACC) strains of Bacillus subtillis, P. aeruginosa and Serratia marcescens used here were provided from Korean National Genetic Resources Center.
PY - 2014/1/20
Y1 - 2014/1/20
N2 - We have isolated 576 endophytic bacteria from the leaves, stems, and roots of 10 rice cultivars and identified 12 of them as diazotrophic bacteria using a specific primer set of nif gene. Through 16S rDNA sequence analysis, nifH genes were confirmed in the two species of Penibacillus, three species of Microbacterium, three Bacillus species, and four species of Klebsiella. Rice seeds treated with these plant growth-promoting bacteria (PGPB) showed improved plant growth, increased height and dry weight and antagonistic effects against fungal pathogens. In addition, auxin and siderophore producing ability, and phosphate solubilizing activity were studied for the possible mechanisms of plant growth promotion. Among 12 isolates tested, 10 strains have shown higher auxin producing activity, 6 isolates were confirmed as strains with high siderophore producing activity while 4 isolates turned out to have high phosphate-solubilizing activity. These results strongly suggest that the endophytic diazotrophic bacteria characterized in this study could be successfully used to promote plant growth and inducing fungal resistance in plants.
AB - We have isolated 576 endophytic bacteria from the leaves, stems, and roots of 10 rice cultivars and identified 12 of them as diazotrophic bacteria using a specific primer set of nif gene. Through 16S rDNA sequence analysis, nifH genes were confirmed in the two species of Penibacillus, three species of Microbacterium, three Bacillus species, and four species of Klebsiella. Rice seeds treated with these plant growth-promoting bacteria (PGPB) showed improved plant growth, increased height and dry weight and antagonistic effects against fungal pathogens. In addition, auxin and siderophore producing ability, and phosphate solubilizing activity were studied for the possible mechanisms of plant growth promotion. Among 12 isolates tested, 10 strains have shown higher auxin producing activity, 6 isolates were confirmed as strains with high siderophore producing activity while 4 isolates turned out to have high phosphate-solubilizing activity. These results strongly suggest that the endophytic diazotrophic bacteria characterized in this study could be successfully used to promote plant growth and inducing fungal resistance in plants.
KW - Diazotrophic bacteria
KW - Endophytic
KW - Nitrogen-fixing
KW - Plant growth-promoting bacteria (PGPB)
KW - Siderophore
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U2 - 10.1016/j.micres.2013.06.003
DO - 10.1016/j.micres.2013.06.003
M3 - Article
C2 - 23871145
AN - SCOPUS:84889098512
SN - 0944-5013
VL - 169
SP - 83
EP - 98
JO - Microbiological Research
JF - Microbiological Research
IS - 1
ER -