Isolation and characterization of proSS1-32, a peptide derived from the N-terminal region of porcine preprosomatostatin

Wolfgang E. Schmidt; Viktor Mutt; Hartmut Kratzin; Mats Carlquist; J. Michael Conlon; Werner Creutzfeldt

doi:10.1016/0014-5793(85)80060-0

Isolation and characterization of proSS_1-32, a peptide derived from the N-terminal region of porcine preprosomatostatin

Wolfgang E. Schmidt, Viktor Mutt, Hartmut Kratzin, Mats Carlquist, J. Michael Conlon, Werner Creutzfeldt

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A peptide derived from the N-terminal region of porcine prosomatostatin, proSS_1-32, has been purified to homogeneity from extracts of porcine upper intestine. Amino acid analysis revealed that the peptide consists of 32 residues. The complete primary structure was determined as: A P S D P R L R Q F L Q K S L A A A A G K Q E L A K Y F L A E L This sequence obviously comprises residues 1-32 of porcine prosomatostatin since it is identical to the corresponding sequence in human preprosomatostatin. The postulated cleavage site in porcine prosomatostatin is a Leu-Leu bond between residues 32 and 33, thus confirming previous studies of the processing of the somatostatin precursor in the rat and transgenic mouse.

Original language	English
Pages (from-to)	141-146
Number of pages	6
Journal	FEBS Letters
Volume	192
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(85)80060-0
Publication status	Published - Nov 11 1985
Externally published	Yes

Keywords

Gastrointestinal peptide
Isolation
N-terminal prosomatostatin peptide
Porcine gut extract
Primary structure Preprosomatostatin processing

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(85)80060-0

Cite this

@article{96373cfda78e48479486f1819e5d6fef,

title = "Isolation and characterization of proSS1-32, a peptide derived from the N-terminal region of porcine preprosomatostatin",

abstract = "A peptide derived from the N-terminal region of porcine prosomatostatin, proSS1-32, has been purified to homogeneity from extracts of porcine upper intestine. Amino acid analysis revealed that the peptide consists of 32 residues. The complete primary structure was determined as: A P S D P R L R Q F L Q K S L A A A A G K Q E L A K Y F L A E L This sequence obviously comprises residues 1-32 of porcine prosomatostatin since it is identical to the corresponding sequence in human preprosomatostatin. The postulated cleavage site in porcine prosomatostatin is a Leu-Leu bond between residues 32 and 33, thus confirming previous studies of the processing of the somatostatin precursor in the rat and transgenic mouse.",

keywords = "Gastrointestinal peptide, Isolation, N-terminal prosomatostatin peptide, Porcine gut extract, Primary structure Preprosomatostatin processing",

author = "Schmidt, {Wolfgang E.} and Viktor Mutt and Hartmut Kratzin and Mats Carlquist and Conlon, {J. Michael} and Werner Creutzfeldt",

note = "Funding Information: We thank Professor N. Hilschmann for generous support in performing amino acid analysisa nd J. Friedrich for help with the liquid-phases equencingE. xcellentt echnicala ssistancbey MS E. Rabbe,M S D. Bonhaus,M S D. Michels, MS B. Wehle, Mrs L. Melin and Mrs B. Agerberthi s gratefully acknowledged. This study was supported by Deutsche Forschungsgemeinschaft (grant Cr 20/19-4),t he SwedishM edical Research Council {grant1 3X-01010)S, tiftung Volkswagen-werk and Smith Kline DauelsbergF oundation.",

year = "1985",

month = nov,

day = "11",

doi = "10.1016/0014-5793(85)80060-0",

language = "English",

volume = "192",

pages = "141--146",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - Isolation and characterization of proSS1-32, a peptide derived from the N-terminal region of porcine preprosomatostatin

AU - Schmidt, Wolfgang E.

AU - Mutt, Viktor

AU - Kratzin, Hartmut

AU - Carlquist, Mats

AU - Conlon, J. Michael

AU - Creutzfeldt, Werner

N1 - Funding Information: We thank Professor N. Hilschmann for generous support in performing amino acid analysisa nd J. Friedrich for help with the liquid-phases equencingE. xcellentt echnicala ssistancbey MS E. Rabbe,M S D. Bonhaus,M S D. Michels, MS B. Wehle, Mrs L. Melin and Mrs B. Agerberthi s gratefully acknowledged. This study was supported by Deutsche Forschungsgemeinschaft (grant Cr 20/19-4),t he SwedishM edical Research Council {grant1 3X-01010)S, tiftung Volkswagen-werk and Smith Kline DauelsbergF oundation.

PY - 1985/11/11

Y1 - 1985/11/11

N2 - A peptide derived from the N-terminal region of porcine prosomatostatin, proSS1-32, has been purified to homogeneity from extracts of porcine upper intestine. Amino acid analysis revealed that the peptide consists of 32 residues. The complete primary structure was determined as: A P S D P R L R Q F L Q K S L A A A A G K Q E L A K Y F L A E L This sequence obviously comprises residues 1-32 of porcine prosomatostatin since it is identical to the corresponding sequence in human preprosomatostatin. The postulated cleavage site in porcine prosomatostatin is a Leu-Leu bond between residues 32 and 33, thus confirming previous studies of the processing of the somatostatin precursor in the rat and transgenic mouse.

AB - A peptide derived from the N-terminal region of porcine prosomatostatin, proSS1-32, has been purified to homogeneity from extracts of porcine upper intestine. Amino acid analysis revealed that the peptide consists of 32 residues. The complete primary structure was determined as: A P S D P R L R Q F L Q K S L A A A A G K Q E L A K Y F L A E L This sequence obviously comprises residues 1-32 of porcine prosomatostatin since it is identical to the corresponding sequence in human preprosomatostatin. The postulated cleavage site in porcine prosomatostatin is a Leu-Leu bond between residues 32 and 33, thus confirming previous studies of the processing of the somatostatin precursor in the rat and transgenic mouse.

KW - Gastrointestinal peptide

KW - Isolation

KW - N-terminal prosomatostatin peptide

KW - Porcine gut extract

KW - Primary structure Preprosomatostatin processing

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