TY - JOUR
T1 - Isolation and structural characterization of two novel peptides derived from proopiomelanocortin in the pituitary of the rainbow trout
AU - Tollemer, Hélène
AU - Leprince, Jérôme
AU - Galas, Ludovic
AU - Vandesande, Frans
AU - Mevel, Jean Claude Le
AU - Tonon, Marie Christine
AU - Conlon, J. Michael
AU - Vaudry, Hubert
N1 - Funding Information:
This work was supported by grants from INSERM (U 413), EU Human Capital and Mobility (Grant ERBCHRXCT 92-0017), NSF (IBN-84 188190) and the Conseil ReÂgional de Haute-Normandie. The authors wish to thank Dr Per F. Nielsen, Novo-Nordisk (Bagsvaerd, Denmark) for mass spectometry measurement. H.T. was a recipient of a fellowship from the EU Human Capital and Mobility Program. J.L. was a recipient of a fellowship from ORIL laboratories and the Conseil ReÂgional de Haute-Normandie. L.G. was a recipient of a fellowship from the MinisteÁre de l'Enseignement SupeÂrieur et de la Recherche.
PY - 1997/9/18
Y1 - 1997/9/18
N2 - The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.
AB - The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.
UR - https://www.scopus.com/pages/publications/0031577578
UR - https://www.scopus.com/pages/publications/0031577578#tab=citedBy
U2 - 10.1006/bbrc.1997.7356
DO - 10.1006/bbrc.1997.7356
M3 - Article
C2 - 9299569
AN - SCOPUS:0031577578
SN - 0006-291X
VL - 238
SP - 653
EP - 657
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -