Kinetics of the enzymatic hydrolysis of palm oil by lipase

Sulaiman Al-Zuhair, Masitah Hasan, K. B. Ramachandran

Research output: Contribution to journalArticlepeer-review

119 Citations (Scopus)


The kinetics of the enzymic hydrolysis of palm oil using lipase in a batch reactor has been investigated. The lipase enzyme used was not ester bond position selective and its activity at the interface was higher compared to that in the bulk. A mathematical model taking into account the mechanism of the hydrolysis reaction and the effect of interfacial area between the oil phase and the aqueous phase containing the enzyme was developed. A correlation between the interfacial area and the operating conditions including agitation speed and oil volume fraction was established experimentally. The kinetic parameters were estimated by fitting the data to the model and comparing with previously reported values. The kinetic model represented the experimental data accurately.

Original languageEnglish
Pages (from-to)1155-1163
Number of pages9
JournalProcess Biochemistry
Issue number8
Publication statusPublished - Mar 28 2003
Externally publishedYes


  • Interfacial area
  • Kinetic model
  • Lipase hydrolysis
  • Palm oil

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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