Large scale serial two-photon microscopy to investigate local vascular changes in whole rodent brain models of Alzheimer's disease

P. Delafontaine-Martel, J. Lefebvre, R. Damseh, A. Castonguay, P. Tardif, F. Lesage

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In this study, an automated serial two-photon microscope was used to image a fluorescent gelatin filled rodent's brain in 3D. A method to compute vascular density using automatic segmentation was combined with coregistration techniques to build group-level vasculature metrics. By studying the medial prefrontal cortex and the hippocampal formation of 3 age groups (2, 4.5 and 8 months old), we compared vascular density for both WT and an Alzheimer model transgenic brain (APP/PS1). We observe a loss of vascular density caused by the ageing process and we propose further analysis to confirm our results.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVIII
EditorsAmmasi Periasamy, Xiaoliang S. Xie, Xiaoliang S. Xie, Karsten Konig, Peter T. C. So
PublisherSPIE
ISBN (Electronic)9781510614819
DOIs
Publication statusPublished - 2018
Externally publishedYes
EventMultiphoton Microscopy in the Biomedical Sciences XVIII 2018 - San Francisco, United States
Duration: Jan 28 2018Jan 30 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10498
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XVIII 2018
Country/TerritoryUnited States
CitySan Francisco
Period1/28/181/30/18

Keywords

  • Alzheimer's disease
  • Serial imaging
  • Two-photon microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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