Lc-ms phytochemical screening, in vitro antioxidant, antimicrobial and anticancer activity of microalgae nannochloropsis oculata extract

Adil Farooq Wali, Yusra Al Dhaheri, Jayachithra Ramakrishna Pillai, Ahlam Mushtaq, Padma G.M. Rao, Syed Arman Rabbani, Aimen Firdous, Mohamed Soliman Elshikh, Dunia A. Al Farraj

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Nowadays, marine microalgae are recognized to be a considerably novel and rich origin of bioactive moieties utilized in the sectors of nutraceuticals and pharmaceuticals. In the present study, Nannochloropsis oculata extract (AME) was associated with a wide variety of pharmacological studies such as in vitro antioxidant, antibacterial, and antifungal and anticancer activity (MDA-MB-231) in cancer cells through in vitro models. In the study, the chemical composition and structure of the bioactive compounds found in the AME extract were studied using the LC-MS technique. The results of the anticancer activity showed a decrease in the percentage of cell viability of the MDA-MB-231 cells in a concentration-and time-dependent manner (400 µg/mL at 24 h, 300 µg/mL at 48 h, and 200 µg/mL at 72 h). We have also observed morphological changes in the cells that could be associated with treatment with AME extract. Our observation of the AME extract-treated MDA-MB231 cells under light microscopy showed that when the concentration increased, the number of cells began to decrease. As far as LC-MS analysis is concerned, it showed the presence of the bioactive molecules was terpenoids along with carotenoids, polyphenolic and fatty acids. The result revealed that the AME extract exhibited noteworthy in vitro free radical scavenging potential, with an IC50 value of 52.10 ± 0.85 µg/L in DPPH assay, 122.84 ± 2.32 µg/mL in H2O2 assay and, 96.95 ± 1.68 µg/mL in ABTS assay. The activity was found to be highly significant against bacteria (Gram-positive and negative) and moderately significant against fungal strain with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)/minimum fungicidal concentration (MFC) values between 15.63 and 500 µg/mL.

Original languageEnglish
Article number54
Pages (from-to)1-11
Number of pages11
Issue number4
Publication statusPublished - Dec 2020


  • Antimicrobial
  • Antioxidants assay
  • MDA-MB-231 cells
  • Nannochloropsis oculata
  • Polyphenols

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation


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