Lipase-catalyzed synthesis of cocoa butter equivalent from palm olein and saturated fatty acid distillate from palm oil physical refinery

Ibrahim O. Mohamed

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Cocoa butter equivalent was prepared by enzymatic acidolysis reaction of substrate consisting of refined palm olein oil and palmitic-stearic fatty acid mixture. The reactions were performed in a batch reactor at a temperature of 60 °C in an orbital shaker operated at 160 RPM. Different mass ratios of substrates were explored and the compositions of the five major triacylglycerol (TAG) of the structured lipids were identified and quantified using cocoa butter-certified reference material IRMM-801. The reaction resulted in production of cococa butter equivent with TAG compostion (POP 26.6 %, POS 42.1, POO 7.5, SOS 18.0 %, and SOO 5.8 %) and melting temperature between 34.7 and 39.6 °C which is close to that of the cocoa butter. The result of this research demonstrated the potential use of saturated fatty acid distillate (palmitic and stearic fatty acids) obtained from palm oil physical refining process into a value-added product.

    Original languageEnglish
    Pages (from-to)1405-1415
    Number of pages11
    JournalApplied Biochemistry and Biotechnology
    Volume168
    Issue number6
    DOIs
    Publication statusPublished - Nov 2012

    Keywords

    • Acidolysis
    • Cocoa butter equivalent
    • Interesterification
    • Lipase
    • Palm oil
    • Structured lipids
    • Triacylglycerol

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology

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