Lipase Immobilization on Macroporous ZIF-8 for Enhanced Enzymatic Biodiesel Production

Yingli Hu, Hao Zhou, Lingmei Dai, Dehua Liu, Sulaiman Al-Zuhair, Wei Du

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Immobilization of enzyme on metal-organic frameworks (MOFs) has drawn increasing interest owing to their many well-recognized characteristics. However, the pore sizes of MOFs (mostly micropores and mesopores) limit their application for enzyme immobilization to a great extent owing to the large size of enzyme molecules. Synthesis of MOFs with macropores would therefore solve this problem, typically encountered with conventional MOFs. In this work, macroporous zeolitic imidazolate frameworks (ZIF-8), referred to as M-ZIF-8, were synthesized and used for immobilization of Aspergillus niger lipase (ANL). Immobilization efficiency using M-ZIF-8 and enzymatic catalytic performance for biodiesel preparation were investigated. The immobilized ANL on M-ZIF-8 (ANL@M-ZIF-8) showed higher enzymatic activity (6.5-fold), activity recovery (3.8-fold), thermal stability (1.4-and 3.4-fold at 80 and 100 °C, respectively), reusability (after five cycles, 68% of initial activity was maintained), and porosity than ANL on conventional ZIF-8 (ANL/ZIF-8). In addition, by using ANL@M-ZIF-8 for catalyzing a biodiesel production reaction, a higher fatty acid methyl ester yield was achieved.

Original languageEnglish
Pages (from-to)2143-2148
Number of pages6
JournalACS Omega
Issue number3
Publication statusPublished - Jan 26 2021

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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