Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

Yan Sheng; Zena Huang; Tenghua Zhang; Feiyang Qian; Yuqing Zhu; Zaizai Dong; Qingyang Zhang; Qi Lei; Fanhui Kong; Yuqiong Wang; Maddie Walden; Stefan Wuttke; Lingqian Chang; Wei Zhu; Jiaming Hu

doi:10.1021/jacs.2c02268

Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

Yan Sheng, Zena Huang, Tenghua Zhang, Feiyang Qian, Yuqing Zhu, Zaizai Dong, Qingyang Zhang, Qi Lei, Fanhui Kong, Yuqiong Wang, Maddie Walden, Stefan Wuttke, Lingqian Chang, Wei Zhu, Jiaming Hu

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.

Original language	English
Pages (from-to)	9443-9450
Number of pages	8
Journal	Journal of the American Chemical Society
Volume	144
Issue number	21
DOIs	https://doi.org/10.1021/jacs.2c02268
Publication status	Published - Jun 1 2022
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/jacs.2c02268

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abstract = "Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.",

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T1 - Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

AU - Sheng, Yan

AU - Huang, Zena

AU - Zhang, Tenghua

AU - Qian, Feiyang

AU - Zhu, Yuqing

AU - Dong, Zaizai

AU - Zhang, Qingyang

AU - Lei, Qi

AU - Kong, Fanhui

AU - Wang, Yuqiong

AU - Walden, Maddie

AU - Wuttke, Stefan

AU - Chang, Lingqian

AU - Zhu, Wei

AU - Hu, Jiaming

PY - 2022/6/1

Y1 - 2022/6/1

N2 - Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.

AB - Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.

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DO - 10.1021/jacs.2c02268

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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ER -

Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

Abstract

ASJC Scopus subject areas

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