Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

Yan Sheng; Zena Huang; Tenghua Zhang; Feiyang Qian; Yuqing Zhu; Zaizai Dong; Qingyang Zhang; Qi Lei; Fanhui Kong; Yuqiong Wang; Maddie Walden; Stefan Wuttke; Lingqian Chang; Wei Zhu; Jiaming Hu

doi:10.1021/jacs.2c02268

Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

Yan Sheng
, Zena Huang
, Tenghua Zhang
, Feiyang Qian
, Yuqing Zhu
, Zaizai Dong
, Qingyang Zhang
, Qi Lei
, Fanhui Kong
, Yuqiong Wang
, Maddie Walden
, Stefan Wuttke
, Lingqian Chang
, Wei Zhu
, Jiaming Hu

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.

Original language	English
Pages (from-to)	9443-9450
Number of pages	8
Journal	Journal of the American Chemical Society
Volume	144
Issue number	21
DOIs	https://doi.org/10.1021/jacs.2c02268
Publication status	Published - Jun 1 2022
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/jacs.2c02268

Cite this

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abstract = "Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.",

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T1 - Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

AU - Sheng, Yan

AU - Huang, Zena

AU - Zhang, Tenghua

AU - Qian, Feiyang

AU - Zhu, Yuqing

AU - Dong, Zaizai

AU - Zhang, Qingyang

AU - Lei, Qi

AU - Kong, Fanhui

AU - Wang, Yuqiong

AU - Walden, Maddie

AU - Wuttke, Stefan

AU - Chang, Lingqian

AU - Zhu, Wei

AU - Hu, Jiaming

PY - 2022/6/1

Y1 - 2022/6/1

N2 - Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.

AB - Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.

UR - https://www.scopus.com/pages/publications/85131215561

UR - https://www.scopus.com/pages/publications/85131215561#tab=citedBy

U2 - 10.1021/jacs.2c02268

DO - 10.1021/jacs.2c02268

M3 - Article

C2 - 35603745

AN - SCOPUS:85131215561

SN - 0002-7863

VL - 144

SP - 9443

EP - 9450

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 21

ER -

Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies

Abstract

ASJC Scopus subject areas

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