Loss of muscarinic M4 receptors in spinal cord of arthritic rats: Implications for a role of M4 receptors in pain response

Ezra Mulugeta; Nahid El-Bakri; Evert Karlsson; Adlan Elhassan; Abdu Adem

doi:10.1016/S0006-8993(03)03025-7

Loss of muscarinic M₄ receptors in spinal cord of arthritic rats: Implications for a role of M₄ receptors in pain response

Ezra Mulugeta, Nahid El-Bakri, Evert Karlsson, Adlan Elhassan, Abdu Adem

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Changes in the levels of muscarinic M₄ receptors in spinal cord of acute and chronic arthritic rats (animal models of pain) were studied by receptor autoradiography using muscarinic M₄ receptor subtype selective ligand. Arthritis was induced in female Lewis rats by single intradermal injection of heat-killed Mycobacterium butyricum and sacrificed 12 days (acute group) and 30 days (chronic and control groups) after induction of arthritis. Our results demonstrate significant reduction in the level of M ₄ receptors in the spinal cord (Rexed laminae I-X) of acute and chronic arthritic rats compared to controls. These findings suggest that the muscarinic M₄ receptor subtype may be involved in cholinergic mechanisms of analgesia.

Original language	English
Pages (from-to)	284-287
Number of pages	4
Journal	Brain Research
Volume	982
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(03)03025-7
Publication status	Published - Aug 29 2003

Keywords

Acetylcholine
Analgesia
Antinociception
Arthritis
Inflammatory pain
Muscarinic receptor subtype

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/S0006-8993(03)03025-7

Cite this

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title = "Loss of muscarinic M4 receptors in spinal cord of arthritic rats: Implications for a role of M4 receptors in pain response",

abstract = "Changes in the levels of muscarinic M4 receptors in spinal cord of acute and chronic arthritic rats (animal models of pain) were studied by receptor autoradiography using muscarinic M4 receptor subtype selective ligand. Arthritis was induced in female Lewis rats by single intradermal injection of heat-killed Mycobacterium butyricum and sacrificed 12 days (acute group) and 30 days (chronic and control groups) after induction of arthritis. Our results demonstrate significant reduction in the level of M 4 receptors in the spinal cord (Rexed laminae I-X) of acute and chronic arthritic rats compared to controls. These findings suggest that the muscarinic M4 receptor subtype may be involved in cholinergic mechanisms of analgesia.",

keywords = "Acetylcholine, Analgesia, Antinociception, Arthritis, Inflammatory pain, Muscarinic receptor subtype",

author = "Ezra Mulugeta and Nahid El-Bakri and Evert Karlsson and Adlan Elhassan and Abdu Adem",

note = "Funding Information: We are grateful to Dr. U. Volpe and Dr. H. Mangat for help with the preparations of illustrations. We are also grateful to Professor Urban Lindgren and Dr. Isam Suliman. This study was supported by grants from the Swedish Medical Council and Alzheimerfonden to A. Adem and E. Karlsson.",

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AU - Mulugeta, Ezra

AU - El-Bakri, Nahid

AU - Karlsson, Evert

AU - Elhassan, Adlan

AU - Adem, Abdu

N1 - Funding Information: We are grateful to Dr. U. Volpe and Dr. H. Mangat for help with the preparations of illustrations. We are also grateful to Professor Urban Lindgren and Dr. Isam Suliman. This study was supported by grants from the Swedish Medical Council and Alzheimerfonden to A. Adem and E. Karlsson.

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N2 - Changes in the levels of muscarinic M4 receptors in spinal cord of acute and chronic arthritic rats (animal models of pain) were studied by receptor autoradiography using muscarinic M4 receptor subtype selective ligand. Arthritis was induced in female Lewis rats by single intradermal injection of heat-killed Mycobacterium butyricum and sacrificed 12 days (acute group) and 30 days (chronic and control groups) after induction of arthritis. Our results demonstrate significant reduction in the level of M 4 receptors in the spinal cord (Rexed laminae I-X) of acute and chronic arthritic rats compared to controls. These findings suggest that the muscarinic M4 receptor subtype may be involved in cholinergic mechanisms of analgesia.

AB - Changes in the levels of muscarinic M4 receptors in spinal cord of acute and chronic arthritic rats (animal models of pain) were studied by receptor autoradiography using muscarinic M4 receptor subtype selective ligand. Arthritis was induced in female Lewis rats by single intradermal injection of heat-killed Mycobacterium butyricum and sacrificed 12 days (acute group) and 30 days (chronic and control groups) after induction of arthritis. Our results demonstrate significant reduction in the level of M 4 receptors in the spinal cord (Rexed laminae I-X) of acute and chronic arthritic rats compared to controls. These findings suggest that the muscarinic M4 receptor subtype may be involved in cholinergic mechanisms of analgesia.

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KW - Inflammatory pain

KW - Muscarinic receptor subtype

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