Lysozyme and DNA binding affinity of Pd(II) and Pt(II) complexes bearing charged: N, N -pyridylbenzimidazole bidentate ligands

Ahmed M. Mansour; Ola R. Shehab

doi:10.1039/c7dt04347c

Lysozyme and DNA binding affinity of Pd(II) and Pt(II) complexes bearing charged: N, N -pyridylbenzimidazole bidentate ligands

Ahmed M. Mansour, Ola R. Shehab

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

New cisplatin analogue Pd(ii) and Pt(ii) complexes bearing charged pyridylbenzimidazole derivatives furnished with either an alkylated sulfonate or a phosphonium side chain were synthesized, fully characterized and tested for their antimicrobial and cytotoxic activities against noncancerous human embryonic kidney cells (HEK293). The interactions with CT-DNA were investigated using UV/vis spectroscopy. Assignment of the electronic transitions was performed with the aid of TDDFT. The complexes showed interesting antifungal activity against Candida albicans and Cryptococcus neoformans. The Pd(ii) complex conjugated with alkylated triphenylphosphonium exhibited higher cytotoxicity (CC₅₀ = 8.932 μg mL^-1, equivalent to 12 nM) than the others. The reactivity towards hen egg white lysozyme (HEWL) was investigated by electrospray ionization mass spectrometry. The formation of DNA- and HEWL-adducts was achieved via the noncovalent and covalent interactions.

Original language	English
Pages (from-to)	3459-3468
Number of pages	10
Journal	Dalton Transactions
Volume	47
Issue number	10
DOIs	https://doi.org/10.1039/c7dt04347c
Publication status	Published - 2018
Externally published	Yes

ASJC Scopus subject areas

Inorganic Chemistry

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title = "Lysozyme and DNA binding affinity of Pd(II) and Pt(II) complexes bearing charged: N, N -pyridylbenzimidazole bidentate ligands",

abstract = "New cisplatin analogue Pd(ii) and Pt(ii) complexes bearing charged pyridylbenzimidazole derivatives furnished with either an alkylated sulfonate or a phosphonium side chain were synthesized, fully characterized and tested for their antimicrobial and cytotoxic activities against noncancerous human embryonic kidney cells (HEK293). The interactions with CT-DNA were investigated using UV/vis spectroscopy. Assignment of the electronic transitions was performed with the aid of TDDFT. The complexes showed interesting antifungal activity against Candida albicans and Cryptococcus neoformans. The Pd(ii) complex conjugated with alkylated triphenylphosphonium exhibited higher cytotoxicity (CC50 = 8.932 μg mL-1, equivalent to 12 nM) than the others. The reactivity towards hen egg white lysozyme (HEWL) was investigated by electrospray ionization mass spectrometry. The formation of DNA- and HEWL-adducts was achieved via the noncovalent and covalent interactions.",

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T2 - N, N -pyridylbenzimidazole bidentate ligands

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AU - Shehab, Ola R.

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2018

Y1 - 2018

N2 - New cisplatin analogue Pd(ii) and Pt(ii) complexes bearing charged pyridylbenzimidazole derivatives furnished with either an alkylated sulfonate or a phosphonium side chain were synthesized, fully characterized and tested for their antimicrobial and cytotoxic activities against noncancerous human embryonic kidney cells (HEK293). The interactions with CT-DNA were investigated using UV/vis spectroscopy. Assignment of the electronic transitions was performed with the aid of TDDFT. The complexes showed interesting antifungal activity against Candida albicans and Cryptococcus neoformans. The Pd(ii) complex conjugated with alkylated triphenylphosphonium exhibited higher cytotoxicity (CC50 = 8.932 μg mL-1, equivalent to 12 nM) than the others. The reactivity towards hen egg white lysozyme (HEWL) was investigated by electrospray ionization mass spectrometry. The formation of DNA- and HEWL-adducts was achieved via the noncovalent and covalent interactions.

AB - New cisplatin analogue Pd(ii) and Pt(ii) complexes bearing charged pyridylbenzimidazole derivatives furnished with either an alkylated sulfonate or a phosphonium side chain were synthesized, fully characterized and tested for their antimicrobial and cytotoxic activities against noncancerous human embryonic kidney cells (HEK293). The interactions with CT-DNA were investigated using UV/vis spectroscopy. Assignment of the electronic transitions was performed with the aid of TDDFT. The complexes showed interesting antifungal activity against Candida albicans and Cryptococcus neoformans. The Pd(ii) complex conjugated with alkylated triphenylphosphonium exhibited higher cytotoxicity (CC50 = 8.932 μg mL-1, equivalent to 12 nM) than the others. The reactivity towards hen egg white lysozyme (HEWL) was investigated by electrospray ionization mass spectrometry. The formation of DNA- and HEWL-adducts was achieved via the noncovalent and covalent interactions.

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Lysozyme and DNA binding affinity of Pd(II) and Pt(II) complexes bearing charged: N, N -pyridylbenzimidazole bidentate ligands

Abstract

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