Macrophage apoptosis in rat skeletal muscle treated with bupivacaine hydrochloride: Possible role of MCP-1

Taisuke Horiguchi, Masa Aki Shibata, Yuko Ito, Nabil A.S. Eid, Muneaki Abe, Yoshinori Otsuki

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


The fate of macrophages infiltrating damaged rat skeletal muscle fibers after intramuscular injection of the anesthetic bupivacaine hydrochloride (BPVC) and the possible roles of monocyte chemoattractant protein-1 (MCP-1) were investigated. The number of macrophages reached a maximum level at 2 days after the injection and then gradually decreased. The number of apoptotic cells detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was elevated at 2-4 days and decreased thereafter. In serial sections, TUNEL-positive cells were also immunopositive for RM-4, an antibody specific for identification of macrophages. Electron microscopy further confirmed that it was the macrophages themselves that were undergoing apoptosis. As assessed by reverse transcriptase-polymerase chain reaction (RT-PCR), high levels of MCP-1 mRNA in BPVC-treated muscles were observed and positively correlated with maximum macrophage infiltration. However, the levels of MCP-1 mRNA returned to normal low values coincident with decrease of inflammation and healing of damaged muscle fiber. Local apoptosis of macrophages, under the control of MCP-1, may be involved in healing of BPVC-treated muscles.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalMuscle and Nerve
Issue number1
Publication statusPublished - 2002
Externally publishedYes


  • Apoptosis
  • Local anesthesia
  • Macrophage
  • Monocyte chemoattractant protein-1
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)


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