Magainin-AM2 improves glucose homeostasis and beta cell function in high-fat fed mice

O. O. Ojo; D. K. Srinivasan; B. O. Owolabi; J. M. Conlon; P. R. Flatt; Y. H.A. Abdel-Wahab

doi:10.1016/j.bbagen.2014.10.011

Magainin-AM2 improves glucose homeostasis and beta cell function in high-fat fed mice

O. O. Ojo, D. K. Srinivasan, B. O. Owolabi, J. M. Conlon, P. R. Flatt, Y. H.A. Abdel-Wahab

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Background Magainin-AM2, a previously described amphibian host-defense peptide, stimulates insulin- and glucagon-like peptide-1-release in vitro. This study investigated anti-diabetic effects of the peptide in mice with diet-induced obesity and glucose intolerance.

Methods Male National Institute of Health Swiss mice were maintained on a high-fat diet for 12-weeks prior to the daily treatment with magainin-AM2. Various indices of glucose tolerance were monitored together with insulin secretory responsiveness of islets at conclusion of study.

Results Following twice daily treatment with magainin-AM2 for 15 days, no significant difference in body weight and food intake was observed compared with saline-treated high fat control animals. However, non-fasting blood glucose was significantly (P

Conclusion These results indicate that magainin-AM2 improves glucose tolerance, insulin sensitivity and islet beta cells secretory responsiveness in mice with obesity-diabetes. General significance The activity of magainin-AM2 suggests the possibility of exploiting this peptide for treatment of type 2 diabetes.

Original language	English
Pages (from-to)	80-87
Number of pages	8
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	1850
Issue number	1
DOIs	https://doi.org/10.1016/j.bbagen.2014.10.011
Publication status	Published - Jan 2015
Externally published	Yes

Keywords

Amphibian peptide
Magainin-AM2
Obesity Insulin-release
Type 2 diabetes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/j.bbagen.2014.10.011

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title = "Magainin-AM2 improves glucose homeostasis and beta cell function in high-fat fed mice",

abstract = "Background Magainin-AM2, a previously described amphibian host-defense peptide, stimulates insulin- and glucagon-like peptide-1-release in vitro. This study investigated anti-diabetic effects of the peptide in mice with diet-induced obesity and glucose intolerance.Methods Male National Institute of Health Swiss mice were maintained on a high-fat diet for 12-weeks prior to the daily treatment with magainin-AM2. Various indices of glucose tolerance were monitored together with insulin secretory responsiveness of islets at conclusion of study.Results Following twice daily treatment with magainin-AM2 for 15 days, no significant difference in body weight and food intake was observed compared with saline-treated high fat control animals. However, non-fasting blood glucose was significantly (PConclusion These results indicate that magainin-AM2 improves glucose tolerance, insulin sensitivity and islet beta cells secretory responsiveness in mice with obesity-diabetes. General significance The activity of magainin-AM2 suggests the possibility of exploiting this peptide for treatment of type 2 diabetes.",

keywords = "Amphibian peptide, Magainin-AM2, Obesity Insulin-release, Type 2 diabetes",

author = "Ojo, {O. O.} and Srinivasan, {D. K.} and Owolabi, {B. O.} and Conlon, {J. M.} and Flatt, {P. R.} and Abdel-Wahab, {Y. H.A.}",

note = "Funding Information: Funding for this study was provided by a project grant from Diabetes UK (Project Number: 12/0004457 ) and award of a University of Ulster Vice Chancellor's Research Studentship to DKS. Publisher Copyright: {\textcopyright} 2014 Elsevier B.V. All rights reserved.",

year = "2015",

month = jan,

doi = "10.1016/j.bbagen.2014.10.011",

language = "English",

volume = "1850",

pages = "80--87",

journal = "Biochimica et Biophysica Acta - General Subjects",

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T1 - Magainin-AM2 improves glucose homeostasis and beta cell function in high-fat fed mice

AU - Ojo, O. O.

AU - Srinivasan, D. K.

AU - Owolabi, B. O.

AU - Conlon, J. M.

AU - Flatt, P. R.

AU - Abdel-Wahab, Y. H.A.

N1 - Funding Information: Funding for this study was provided by a project grant from Diabetes UK (Project Number: 12/0004457 ) and award of a University of Ulster Vice Chancellor's Research Studentship to DKS. Publisher Copyright: © 2014 Elsevier B.V. All rights reserved.

PY - 2015/1

Y1 - 2015/1

N2 - Background Magainin-AM2, a previously described amphibian host-defense peptide, stimulates insulin- and glucagon-like peptide-1-release in vitro. This study investigated anti-diabetic effects of the peptide in mice with diet-induced obesity and glucose intolerance.Methods Male National Institute of Health Swiss mice were maintained on a high-fat diet for 12-weeks prior to the daily treatment with magainin-AM2. Various indices of glucose tolerance were monitored together with insulin secretory responsiveness of islets at conclusion of study.Results Following twice daily treatment with magainin-AM2 for 15 days, no significant difference in body weight and food intake was observed compared with saline-treated high fat control animals. However, non-fasting blood glucose was significantly (PConclusion These results indicate that magainin-AM2 improves glucose tolerance, insulin sensitivity and islet beta cells secretory responsiveness in mice with obesity-diabetes. General significance The activity of magainin-AM2 suggests the possibility of exploiting this peptide for treatment of type 2 diabetes.

AB - Background Magainin-AM2, a previously described amphibian host-defense peptide, stimulates insulin- and glucagon-like peptide-1-release in vitro. This study investigated anti-diabetic effects of the peptide in mice with diet-induced obesity and glucose intolerance.Methods Male National Institute of Health Swiss mice were maintained on a high-fat diet for 12-weeks prior to the daily treatment with magainin-AM2. Various indices of glucose tolerance were monitored together with insulin secretory responsiveness of islets at conclusion of study.Results Following twice daily treatment with magainin-AM2 for 15 days, no significant difference in body weight and food intake was observed compared with saline-treated high fat control animals. However, non-fasting blood glucose was significantly (PConclusion These results indicate that magainin-AM2 improves glucose tolerance, insulin sensitivity and islet beta cells secretory responsiveness in mice with obesity-diabetes. General significance The activity of magainin-AM2 suggests the possibility of exploiting this peptide for treatment of type 2 diabetes.

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KW - Magainin-AM2

KW - Obesity Insulin-release

KW - Type 2 diabetes

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Magainin-AM2 improves glucose homeostasis and beta cell function in high-fat fed mice

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