Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

T. E. Adrian, A. J. Bacarese-Hamilton, S. R. Bloom

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalPeptides
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 2 1985
Externally publishedYes

Keywords

  • CCK-8
  • Cholecystokinin octapeptide
  • High pressure liquid chromatography
  • Radioimmunoassay Tyroc

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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