Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

T. E. Adrian; A. J. Bacarese-Hamilton; S. R. Bloom

doi:10.1016/0196-9781(85)90068-3

Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

T. E. Adrian, A. J. Bacarese-Hamilton, S. R. Bloom

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.

Original language	English
Pages (from-to)	11-16
Number of pages	6
Journal	Peptides
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/0196-9781(85)90068-3
Publication status	Published - Jan 2 1985
Externally published	Yes

Keywords

CCK-8
Cholecystokinin octapeptide
High pressure liquid chromatography
Radioimmunoassay Tyroc

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0196-9781(85)90068-3

Cite this

@article{acd238bc01794e8598bed18363737f76,

title = "Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay",

abstract = "A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.",

keywords = "CCK-8, Cholecystokinin octapeptide, High pressure liquid chromatography, Radioimmunoassay Tyroc",

author = "Adrian, {T. E.} and Bacarese-Hamilton, {A. J.} and Bloom, {S. R.}",

year = "1985",

month = jan,

day = "2",

doi = "10.1016/0196-9781(85)90068-3",

language = "English",

volume = "6",

pages = "11--16",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

AU - Adrian, T. E.

AU - Bacarese-Hamilton, A. J.

AU - Bloom, S. R.

PY - 1985/1/2

Y1 - 1985/1/2

N2 - A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.

AB - A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.

KW - CCK-8

KW - Cholecystokinin octapeptide

KW - High pressure liquid chromatography

KW - Radioimmunoassay Tyroc

UR - http://www.scopus.com/inward/record.url?scp=0021945671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021945671&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(85)90068-3

DO - 10.1016/0196-9781(85)90068-3

M3 - Article

C2 - 3991357

AN - SCOPUS:0021945671

SN - 0196-9781

VL - 6

SP - 11

EP - 16

JO - Peptides

JF - Peptides

IS - 1

ER -

Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this