Abstract
A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.
Original language | English |
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Pages (from-to) | 11-16 |
Number of pages | 6 |
Journal | Peptides |
Volume | 6 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2 1985 |
Externally published | Yes |
Keywords
- CCK-8
- Cholecystokinin octapeptide
- High pressure liquid chromatography
- Radioimmunoassay Tyroc
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Endocrinology
- Cellular and Molecular Neuroscience