Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

T. E. Adrian; A. J. Bacarese-Hamilton; S. R. Bloom

doi:10.1016/0196-9781(85)90068-3

Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

T. E. Adrian
, A. J. Bacarese-Hamilton
, S. R. Bloom

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.

Original language	English
Pages (from-to)	11-16
Number of pages	6
Journal	Peptides
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/0196-9781(85)90068-3
Publication status	Published - Jan 2 1985
Externally published	Yes

Keywords

CCK-8
Cholecystokinin octapeptide
High pressure liquid chromatography
Radioimmunoassay Tyroc

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0196-9781(85)90068-3

Cite this

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title = "Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay",

abstract = "A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95\% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.",

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year = "1985",

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doi = "10.1016/0196-9781(85)90068-3",

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T1 - Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

AU - Adrian, T. E.

AU - Bacarese-Hamilton, A. J.

AU - Bloom, S. R.

PY - 1985/1/2

Y1 - 1985/1/2

N2 - A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.

AB - A peptide analogue of CCK-8 (Tyroc) which has a tyrosine in place of the amide group in the C-terminal end, has been used both for raising antisera and for iodination. The antisera produced by immunisation with Tyroc are directed towards the N-terminal end of the CCK-8 molecule. The assay system appears totally specific for the CCK-8 sulphated molecule and shows no significant cross-reaction with other molecular forms of CCK, or with the gastrins. The assay can detect changes between adjacent tubes of 0.25 fmol/tube CCK-8 with 95% confidence. The assay is robust, reliable and reproducible and can be used to measure tissue and plasma levels of CCK-8.

KW - CCK-8

KW - Cholecystokinin octapeptide

KW - High pressure liquid chromatography

KW - Radioimmunoassay Tyroc

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DO - 10.1016/0196-9781(85)90068-3

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C2 - 3991357

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VL - 6

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EP - 16

JO - Peptides

JF - Peptides

IS - 1

ER -

Measurement of cholecystokinin octapeptide using a new specific radioimmunoassay

Abstract

Keywords

ASJC Scopus subject areas

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