Mechanism of ipamorelin-evoked insulin release from the pancreas of normal and diabetic rats

Ernest Adeghate, Abdul Samad Ponery

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To examine the effect of ipamorelin (IPA), a novel pentapeptide with a strong growth hormone releasing potency, on insulin secretion from pancreatic tissue fragments of normal and diabetic rats. Materials and methods: Diabetes mellitus was induced by streptozotocin (60 mg kg-1). Four weeks after the induction of diabetes, pancreatic tissue fragments of normal and diabetic rats were removed and incubated with different concentrations (10-12-10-6 M) of IPA. Insulin release from the pancreas was measured by radioimmunoassay. Results: Ipamorelin evoked significant (p<0.04) increases in insulin secretion from the pancreas of normal and diabetic rats. Either diltiazem or yohimbine or propranolol or a combination of atropine, propranolol and yohimbine inhibited IPA-evoked insulin secretion significantly (p<0.03) from the pancreas of normal and diabetic rats. Atropine caused a significant (p<0.007) reduction in the IPA-induced insulin secretion in diabetic but not in normal rats. Conclusion: IPA stimulates insulin release through the calcium channel and the adrenergic receptor pathways. This is the first study to examine the effect of ipamorelin on insulin secretion in the pancreas.

Original languageEnglish
Pages (from-to)403-406
Number of pages4
JournalNeuroendocrinology Letters
Volume25
Issue number6
Publication statusPublished - Dec 2004

Keywords

  • Cholinergic receptor antagonist
  • Diabetes mellitus
  • Insulin
  • Ipamorelin
  • Pancreas adrenergic receptor antagonists
  • Radioimmunoassay

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems
  • Clinical Neurology
  • Psychiatry and Mental health

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