Mediator, TATA-binding protein, and RNA polymerase ii contribute to low histone occupancy at active gene promoters in yeast

Suraiya A. Ansari; Emily Paul; Sebastian Sommer; Corinna Lieleg; Qiye He; Alexandre Z. Daly; Kara A. Rode; Wesley T. Barber; Laura C. Ellis; Erika LaPorta; Amanda M. Orzechowski; Emily Taylor; Tanner Reeb; Jason Wong; Philipp Korber; Randall H. Morse

doi:10.1074/jbc.M113.529354

Mediator, TATA-binding protein, and RNA polymerase ii contribute to low histone occupancy at active gene promoters in yeast

Suraiya A. Ansari, Emily Paul, Sebastian Sommer, Corinna Lieleg, Qiye He, Alexandre Z. Daly, Kara A. Rode, Wesley T. Barber, Laura C. Ellis, Erika LaPorta, Amanda M. Orzechowski, Emily Taylor, Tanner Reeb, Jason Wong, Philipp Korber, Randall H. Morse

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Background: Promoters of active genes in eukaryotes are typically depleted of histone proteins. Results: Histone eviction from the induced CHA1 promoter is compromised by mutations in transcription factors, and higher histone occupancy is also seen at constitutively active promoters in such mutants. Conclusion: Preinitiation complex formation promotes reduced histone occupancy at active gene promoters. Significance: Preinitiation complex components participate in chromatin remodeling.

Original language	English
Pages (from-to)	14981-14995
Number of pages	15
Journal	Journal of Biological Chemistry
Volume	289
Issue number	21
DOIs	https://doi.org/10.1074/jbc.M113.529354
Publication status	Published - 2014
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Ansari, S. A., Paul, E., Sommer, S., Lieleg, C., He, Q., Daly, A. Z., Rode, K. A., Barber, W. T., Ellis, L. C., LaPorta, E., Orzechowski, A. M., Taylor, E., Reeb, T., Wong, J., Korber, P., & Morse, R. H. (2014). Mediator, TATA-binding protein, and RNA polymerase ii contribute to low histone occupancy at active gene promoters in yeast. Journal of Biological Chemistry, 289(21), 14981-14995. https://doi.org/10.1074/jbc.M113.529354

Ansari, SA, Paul, E, Sommer, S, Lieleg, C, He, Q, Daly, AZ, Rode, KA, Barber, WT, Ellis, LC, LaPorta, E, Orzechowski, AM, Taylor, E, Reeb, T, Wong, J, Korber, P & Morse, RH 2014, 'Mediator, TATA-binding protein, and RNA polymerase ii contribute to low histone occupancy at active gene promoters in yeast', Journal of Biological Chemistry, vol. 289, no. 21, pp. 14981-14995. https://doi.org/10.1074/jbc.M113.529354

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title = "Mediator, TATA-binding protein, and RNA polymerase ii contribute to low histone occupancy at active gene promoters in yeast",

abstract = "Background: Promoters of active genes in eukaryotes are typically depleted of histone proteins. Results: Histone eviction from the induced CHA1 promoter is compromised by mutations in transcription factors, and higher histone occupancy is also seen at constitutively active promoters in such mutants. Conclusion: Preinitiation complex formation promotes reduced histone occupancy at active gene promoters. Significance: Preinitiation complex components participate in chromatin remodeling.",

author = "Ansari, {Suraiya A.} and Emily Paul and Sebastian Sommer and Corinna Lieleg and Qiye He and Daly, {Alexandre Z.} and Rode, {Kara A.} and Barber, {Wesley T.} and Ellis, {Laura C.} and Erika LaPorta and Orzechowski, {Amanda M.} and Emily Taylor and Tanner Reeb and Jason Wong and Philipp Korber and Morse, {Randall H.}",

year = "2014",

doi = "10.1074/jbc.M113.529354",

language = "English",

volume = "289",

pages = "14981--14995",

journal = "Journal of Biological Chemistry",

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T1 - Mediator, TATA-binding protein, and RNA polymerase ii contribute to low histone occupancy at active gene promoters in yeast

AU - Ansari, Suraiya A.

AU - Paul, Emily

AU - Sommer, Sebastian

AU - Lieleg, Corinna

AU - He, Qiye

AU - Daly, Alexandre Z.

AU - Rode, Kara A.

AU - Barber, Wesley T.

AU - Ellis, Laura C.

AU - LaPorta, Erika

AU - Orzechowski, Amanda M.

AU - Taylor, Emily

AU - Reeb, Tanner

AU - Wong, Jason

AU - Korber, Philipp

AU - Morse, Randall H.

PY - 2014

Y1 - 2014

N2 - Background: Promoters of active genes in eukaryotes are typically depleted of histone proteins. Results: Histone eviction from the induced CHA1 promoter is compromised by mutations in transcription factors, and higher histone occupancy is also seen at constitutively active promoters in such mutants. Conclusion: Preinitiation complex formation promotes reduced histone occupancy at active gene promoters. Significance: Preinitiation complex components participate in chromatin remodeling.

AB - Background: Promoters of active genes in eukaryotes are typically depleted of histone proteins. Results: Histone eviction from the induced CHA1 promoter is compromised by mutations in transcription factors, and higher histone occupancy is also seen at constitutively active promoters in such mutants. Conclusion: Preinitiation complex formation promotes reduced histone occupancy at active gene promoters. Significance: Preinitiation complex components participate in chromatin remodeling.

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JO - Journal of Biological Chemistry

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IS - 21

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Mediator, TATA-binding protein, and RNA polymerase ii contribute to low histone occupancy at active gene promoters in yeast

Abstract

ASJC Scopus subject areas

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