Mesoporous silica nanoparticles inhibit cellular respiration

Zhimin Tao; Matthew P. Morrow; Tewodros Asefa; Krishna K. Sharma; Cole Duncan; Abhishek Anan; Harvey S. Penefsky; Jerry Goodisman; Abdul Kader Souid

doi:10.1021/nl080250u

Mesoporous silica nanoparticles inhibit cellular respiration

Zhimin Tao, Matthew P. Morrow, Tewodros Asefa, Krishna K. Sharma, Cole Duncan, Abhishek Anan, Harvey S. Penefsky, Jerry Goodisman, Abdul Kader Souid

Research output: Contribution to journal › Article › peer-review

111 Citations (Scopus)

Abstract

We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O ₂ consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.

Original language	English
Pages (from-to)	1517-1526
Number of pages	10
Journal	Nano Letters
Volume	8
Issue number	5
DOIs	https://doi.org/10.1021/nl080250u
Publication status	Published - May 2008
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl080250u

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title = "Mesoporous silica nanoparticles inhibit cellular respiration",

abstract = "We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.",

author = "Zhimin Tao and Morrow, {Matthew P.} and Tewodros Asefa and Sharma, {Krishna K.} and Cole Duncan and Abhishek Anan and Penefsky, {Harvey S.} and Jerry Goodisman and Souid, {Abdul Kader}",

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T1 - Mesoporous silica nanoparticles inhibit cellular respiration

AU - Tao, Zhimin

AU - Morrow, Matthew P.

AU - Asefa, Tewodros

AU - Sharma, Krishna K.

AU - Duncan, Cole

AU - Anan, Abhishek

AU - Penefsky, Harvey S.

AU - Goodisman, Jerry

AU - Souid, Abdul Kader

PY - 2008/5

Y1 - 2008/5

N2 - We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.

AB - We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.

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JO - Nano Letters

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Mesoporous silica nanoparticles inhibit cellular respiration

Abstract

ASJC Scopus subject areas

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