Mesoporous silica nanoparticles inhibit cellular respiration

Zhimin Tao; Matthew P. Morrow; Tewodros Asefa; Krishna K. Sharma; Cole Duncan; Abhishek Anan; Harvey S. Penefsky; Jerry Goodisman; Abdul Kader Souid

doi:10.1021/nl080250u

Mesoporous silica nanoparticles inhibit cellular respiration

Zhimin Tao
, Matthew P. Morrow
, Tewodros Asefa
, Krishna K. Sharma
, Cole Duncan
, Abhishek Anan
, Harvey S. Penefsky
, Jerry Goodisman
, Abdul Kader Souid

Research output: Contribution to journal › Article › peer-review

113 Citations (Scopus)

Abstract

We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O ₂ consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.

Original language	English
Pages (from-to)	1517-1526
Number of pages	10
Journal	Nano Letters
Volume	8
Issue number	5
DOIs	https://doi.org/10.1021/nl080250u
Publication status	Published - May 2008
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl080250u

Cite this

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title = "Mesoporous silica nanoparticles inhibit cellular respiration",

abstract = "We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.",

author = "Zhimin Tao and Morrow, \{Matthew P.\} and Tewodros Asefa and Sharma, \{Krishna K.\} and Cole Duncan and Abhishek Anan and Penefsky, \{Harvey S.\} and Jerry Goodisman and Souid, \{Abdul Kader\}",

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TY - JOUR

T1 - Mesoporous silica nanoparticles inhibit cellular respiration

AU - Tao, Zhimin

AU - Morrow, Matthew P.

AU - Asefa, Tewodros

AU - Sharma, Krishna K.

AU - Duncan, Cole

AU - Anan, Abhishek

AU - Penefsky, Harvey S.

AU - Goodisman, Jerry

AU - Souid, Abdul Kader

PY - 2008/5

Y1 - 2008/5

N2 - We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.

AB - We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500/ μg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 μg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 μg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.

UR - https://www.scopus.com/pages/publications/46749085146

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U2 - 10.1021/nl080250u

DO - 10.1021/nl080250u

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AN - SCOPUS:46749085146

SN - 1530-6984

VL - 8

SP - 1517

EP - 1526

JO - Nano Letters

JF - Nano Letters

IS - 5

ER -

Mesoporous silica nanoparticles inhibit cellular respiration

Abstract

ASJC Scopus subject areas

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