TY - JOUR
T1 - Metabolism of benzo(a)pyrene, dimethylbenzanthracene and aflatoxin B1 by camel liver microsomes
AU - Raza, H.
AU - Montague, W.
N1 - Funding Information:
HR) for HPLC analyses. We acknowledge the technical help of Mr Mansoor Qureshi in this study. Thanks are also due to Miss Amna Oteiba and the Media Production Unit of the FMHS in preparing this manuscript. A part of this research was supported by Grant 5/93 from the Research Committee of the Faculty of Medicine and Health Sciences, UAE.
PY - 1994/3
Y1 - 1994/3
N2 - The ability of camel liver microsomes to metabolise a range of common environmental carcinogens including benzo(a)pyrene, dimethylbenzanthracene and aflatoxin B1 has been investigated. The camel liver has shown the ability to metabolise benzo(a)pyrene, dimethylbenzanthracene and aflatoxin B1 to a number of metabolites. The major metabolites of benzo(a)pyrene produced by camel liver enzymes were identified as its mono-hydroxy derivatives and suggest that the metabolic detoxification pathways of carcinogen metabolism are predominant in this species. Benzo(a)pyrene metabolising activity in camel liver required NADPH and was inhibited by CO and alpha-naphthoflavone suggesting the involvement of cytochrome P450 in the metabolism of this carcinogen by camel liver. The cytochrome P450-dependent metabolism of carcinogen and other specific substrates such as ethoxyresorufin and ethoxycoumarin, by camel liver enzymes, was about 50% higher than that of rat liver enzymes. The cytochrome P450-dependent metabolism of a variety of carcinogenic and other substrates by camel liver demonstrated that there are multiple forms of cytochrome P450 enzymes involved in the metabolism of a wide array of xenobiotics and pollutants.
AB - The ability of camel liver microsomes to metabolise a range of common environmental carcinogens including benzo(a)pyrene, dimethylbenzanthracene and aflatoxin B1 has been investigated. The camel liver has shown the ability to metabolise benzo(a)pyrene, dimethylbenzanthracene and aflatoxin B1 to a number of metabolites. The major metabolites of benzo(a)pyrene produced by camel liver enzymes were identified as its mono-hydroxy derivatives and suggest that the metabolic detoxification pathways of carcinogen metabolism are predominant in this species. Benzo(a)pyrene metabolising activity in camel liver required NADPH and was inhibited by CO and alpha-naphthoflavone suggesting the involvement of cytochrome P450 in the metabolism of this carcinogen by camel liver. The cytochrome P450-dependent metabolism of carcinogen and other specific substrates such as ethoxyresorufin and ethoxycoumarin, by camel liver enzymes, was about 50% higher than that of rat liver enzymes. The cytochrome P450-dependent metabolism of a variety of carcinogenic and other substrates by camel liver demonstrated that there are multiple forms of cytochrome P450 enzymes involved in the metabolism of a wide array of xenobiotics and pollutants.
KW - Aflatoxin B1
KW - Benzo(a)pyrene
KW - Camel liver microsomes
KW - Carcinogen metabolism
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U2 - 10.1016/1367-8280(94)90065-5
DO - 10.1016/1367-8280(94)90065-5
M3 - Article
C2 - 8061945
AN - SCOPUS:0028220454
SN - 1367-8280
VL - 107
SP - 379
EP - 386
JO - Comparative Biochemistry and Physiology. Part C: Pharmacology
JF - Comparative Biochemistry and Physiology. Part C: Pharmacology
IS - 3
ER -