TY - JOUR
T1 - Metabolism of substance P in human plasma and in the rat circulation
AU - Conlon, J. Michael
AU - Göke, Burkhard
N1 - Funding Information:
We wish to thank the Volkswagen-Stiftungfo r financial support.
PY - 1984/7/27
Y1 - 1984/7/27
N2 - Incubation of substance P in human plasma at 37°C resulted in rapid conversion to des (Arg1Pro2) substance P (fragment 3-11) and to des (Arg1-Pro2-Lys3-Pro4) substance P (fragment 5-11). The metabolites were purified by high-performance liquid chromatography and identified by sequence analysis. These data are consistent with the hypothesis that substance P is metabolized by enzyme(s) with the specifity of dipeptidyl aminopeptidase IV (EC 3.1.14.5). Analysis by high-performance liquid chromatography of plasma extracts following intravenous infusion of substance P (300-350 nmoles) into anaesthetized rats showed that the peptide was cleared from the circulation within 1-2 minutes. No circulating metabolites could be identified.
AB - Incubation of substance P in human plasma at 37°C resulted in rapid conversion to des (Arg1Pro2) substance P (fragment 3-11) and to des (Arg1-Pro2-Lys3-Pro4) substance P (fragment 5-11). The metabolites were purified by high-performance liquid chromatography and identified by sequence analysis. These data are consistent with the hypothesis that substance P is metabolized by enzyme(s) with the specifity of dipeptidyl aminopeptidase IV (EC 3.1.14.5). Analysis by high-performance liquid chromatography of plasma extracts following intravenous infusion of substance P (300-350 nmoles) into anaesthetized rats showed that the peptide was cleared from the circulation within 1-2 minutes. No circulating metabolites could be identified.
UR - http://www.scopus.com/inward/record.url?scp=0021284413&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021284413&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)96417-9
DO - 10.1016/S0021-9673(01)96417-9
M3 - Article
C2 - 6207188
AN - SCOPUS:0021284413
SN - 0021-9673
VL - 296
SP - 241
EP - 247
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -