Metabolomics approach for the identification of bioactive compounds released from young and mature soybean upon in vitro gastrointestinal digestion and their effect on health-related bioactive properties

Feyisola Fisayo Ajayi, Amna Alnuaimi, Marwa Hamdi, Hussein Mostafa, Masataka Wakayama, Priti Mudgil, Sajid Maqsood

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The aim of the present study was to comparatively investigate the relative phytochemical profiles (phenolic content, organic and amino acids, free sugars, and other metabolites using metabolomics approach), and bioactive potentials of young (YS) and mature soybean (MS) upon in vitro simulated gastrointestinal digestion (SGID). Cumulatively, a total of 198 metabolites were identified in MS and YS, 119 metabolites in undigested YS, and a total of 136 metabolites in undigested MS, which further increased to 156 and 152 in YS and MS upon SGID, respectively. Gastric digesta of both YS and MS exhibited higher inhibitory properties towards α-amylase and DPP-IV enzymes than their intestinal digesta. Furthermore, the intestinal digesta of MS showed higher antioxidant and anti-inflammatory activities compared to the YS intestinal digesta. Overall, the results suggested that the gastrointestinal digestion of YS and MS displayed distinctive metabolic profiles together with varied bioactive potentials.

Original languageEnglish
Article number136050
JournalFood Chemistry
Volume420
DOIs
Publication statusPublished - Sept 15 2023

Keywords

  • Antioxidant
  • Enzymes inhibitory potentials
  • In vitro gastrointestinal digestion
  • Metabolome profiling
  • Phenolic content
  • Soybean

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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