Micro, Planar-Form Lactate Biosensor for Biomedical Applications

Sayed A. Marzouk, Vasile V. Cosofret, Richard P. Buck, Hua Yang, Wayne E. Cascio, Saad S.M. Hassan

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Lactic acid is an important metabolic product formed during anaerobic glycolysis. There are no continuous methods to measure the extracellular lactate accumulation occurring in the absence of myocardial perfusion. Two forms of biosensors have been constructed. The first miniature version fulfills the operational requirements, such as flexibility, wide linear response range, high selectivity, and fast response time. This biosensor is based on immobilized lactate oxidase with anodic detection of the produced H2O2. An inner layer, which allows selective detection of hydrogen peroxide, is formed by electropolymerization of m-phenylenediamine. A diffusional barrier of polyurethane greatly increases the linear response range. The second miniature sensor uses an anode made of tetrathiofulvalene-tetracyanoquinodimethane (TTF-TCNQ) charge transfer complex. The former sensor was used successfully under reduced ambient oxygen tension (PO2 > 24mm Hg); while the second can operate in the absence of oxygen. The applications are measurements of lactate in the ischemic rabbit papillary muscle under no-flow conditions.

Original languageEnglish
Pages (from-to)66-77
Number of pages12
JournalACS Symposium Series
Publication statusPublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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