TY - JOUR
T1 - Micro, Planar-Form Lactate Biosensor for Biomedical Applications
AU - Marzouk, Sayed A.
AU - Cosofret, Vasile V.
AU - Buck, Richard P.
AU - Yang, Hua
AU - Cascio, Wayne E.
AU - Hassan, Saad S.M.
PY - 1998
Y1 - 1998
N2 - Lactic acid is an important metabolic product formed during anaerobic glycolysis. There are no continuous methods to measure the extracellular lactate accumulation occurring in the absence of myocardial perfusion. Two forms of biosensors have been constructed. The first miniature version fulfills the operational requirements, such as flexibility, wide linear response range, high selectivity, and fast response time. This biosensor is based on immobilized lactate oxidase with anodic detection of the produced H2O2. An inner layer, which allows selective detection of hydrogen peroxide, is formed by electropolymerization of m-phenylenediamine. A diffusional barrier of polyurethane greatly increases the linear response range. The second miniature sensor uses an anode made of tetrathiofulvalene-tetracyanoquinodimethane (TTF-TCNQ) charge transfer complex. The former sensor was used successfully under reduced ambient oxygen tension (PO2 > 24mm Hg); while the second can operate in the absence of oxygen. The applications are measurements of lactate in the ischemic rabbit papillary muscle under no-flow conditions.
AB - Lactic acid is an important metabolic product formed during anaerobic glycolysis. There are no continuous methods to measure the extracellular lactate accumulation occurring in the absence of myocardial perfusion. Two forms of biosensors have been constructed. The first miniature version fulfills the operational requirements, such as flexibility, wide linear response range, high selectivity, and fast response time. This biosensor is based on immobilized lactate oxidase with anodic detection of the produced H2O2. An inner layer, which allows selective detection of hydrogen peroxide, is formed by electropolymerization of m-phenylenediamine. A diffusional barrier of polyurethane greatly increases the linear response range. The second miniature sensor uses an anode made of tetrathiofulvalene-tetracyanoquinodimethane (TTF-TCNQ) charge transfer complex. The former sensor was used successfully under reduced ambient oxygen tension (PO2 > 24mm Hg); while the second can operate in the absence of oxygen. The applications are measurements of lactate in the ischemic rabbit papillary muscle under no-flow conditions.
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U2 - 10.1021/bk-1998-0690.ch006
DO - 10.1021/bk-1998-0690.ch006
M3 - Article
AN - SCOPUS:0346544613
SN - 0097-6156
VL - 690
SP - 66
EP - 77
JO - ACS Symposium Series
JF - ACS Symposium Series
ER -