TY - JOUR
T1 - Microdissection of pig chromosomes
T2 - Dissection of whole chromosomes, arms and bands for construction of paints and libraries
AU - Chaudhary, R.
AU - Kijas, J.
AU - Raudsepp, T.
AU - Guan, X. Y.
AU - Zhang, H.
AU - Chowdhary, B. P.
PY - 1998
Y1 - 1998
N2 - Chromosome microdissection is an important means to efficiently generate a large number of markers from a desired region of a genome. The present study was designed to initiate microdissection and amplification of DNA from whole chromosomes, arms, or bands of porcine chromosomes. The following pig (SSC) chromosomes/segments were scraped: SSC1p, SSC1q26-q2.13, SSC2q11-q14, SSC4q12-q25, SSC13, SSC13q12-q31, SSC13q32-q43, SSC13q32-q43, SSC15, and SSC16q21-q23. After amplification and PCR-labelling, the DNA from the dissected segments were painted back to normal metaphase chromosomes to test their identity. Microdissection of some of the segments (on SSC4, 13 and 15) coincides with the mapping of economically important traits. As a first step towards generation of markers, microloning of amplified product from SSC1p and SSC15 was carried out. The libraries were screened with a (GT)15 oligonucleotideprobe. Future prospects of such a work in farm animals are discussed.
AB - Chromosome microdissection is an important means to efficiently generate a large number of markers from a desired region of a genome. The present study was designed to initiate microdissection and amplification of DNA from whole chromosomes, arms, or bands of porcine chromosomes. The following pig (SSC) chromosomes/segments were scraped: SSC1p, SSC1q26-q2.13, SSC2q11-q14, SSC4q12-q25, SSC13, SSC13q12-q31, SSC13q32-q43, SSC13q32-q43, SSC15, and SSC16q21-q23. After amplification and PCR-labelling, the DNA from the dissected segments were painted back to normal metaphase chromosomes to test their identity. Microdissection of some of the segments (on SSC4, 13 and 15) coincides with the mapping of economically important traits. As a first step towards generation of markers, microloning of amplified product from SSC1p and SSC15 was carried out. The libraries were screened with a (GT)15 oligonucleotideprobe. Future prospects of such a work in farm animals are discussed.
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U2 - 10.1111/j.1601-5223.1998.00265.x
DO - 10.1111/j.1601-5223.1998.00265.x
M3 - Article
C2 - 9760875
AN - SCOPUS:0031670345
SN - 0018-0661
VL - 128
SP - 265
EP - 271
JO - Hereditas
JF - Hereditas
IS - 3
ER -