Microfluidic immunoprecipitation for post-translational modified protein purification

Hui Xia, Bobby Mathew, Tom John, Hisham Hegab, June Feng

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We here report an antibody functionalized microimmunoprecipitation (μIP) method used for enrich lowabundant post-translational modified (PTM) proteins. The device is a fabricated by inert, nontoxic and disposable polydimethylsiloxane (PDMS) using a silane-based chemical modification protocol, which yield an antibody-terminated PDMS surfaces. In this study, the μIP device is specifically designed for purification of carbonylated protein, a representative example here to illustrate the potential applications for any other PTMs, which could be immuno-tagged by specific antibodies. The test model- in vitro oxidized Bovine Serum Albumin (BSA) was first derivitized by dinitrophenylhydrazide (DNPH) and then captured by the anti-DNP immobilized on this μIP device. The surface functional group mapping was systematically analyzed and validated by fluorescence microscopy. Quantitative study of DNP-derivatized carbonylated protein capture recovery and elution efficiency of the device was also studied. We also envision that this proteome enrichment μIP device can be assembled with other lab-on-a-chip components, such as microelectrophoresis or micro-chromatographic devices for follow-up protein analysis. This selective enrichment of modified proteins greatly facilitates the study of low abundant protein biomarkers discovery.

Original languageEnglish
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Number of pages4
Publication statusPublished - 2013
Externally publishedYes
EventWorld Congress on Medical Physics and Biomedical Engineering - Beijing, China
Duration: May 26 2012May 31 2012

Publication series

NameIFMBE Proceedings
Volume39 IFMBE
ISSN (Print)1680-0737


ConferenceWorld Congress on Medical Physics and Biomedical Engineering


  • Micro- Immunoprecipitation
  • Microfluidic Device
  • Polydimethylsiloxane
  • Post-Translational Modification

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering


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