TY - JOUR
T1 - Molecular and immunohistochemical detection of assemblage E, Giardia duodenalis in scouring North Dakota calves
AU - Barigye, Robert
AU - Dyer, Neil W.
AU - Newell, Teresa K.
AU - Khaitsa, Margaret L.
AU - Trout, James M.
AU - Santin, Monica
AU - Fayer, Ronald
N1 - Funding Information:
The authors wish to thank Ms. Ev Schamber for her expert technical assistance. This study was funded through a grant from the North Dakota State Board of Agricultural Research and Education (SBARE), Project No. FARG090069.
PY - 2008/11/7
Y1 - 2008/11/7
N2 - Despite many reports on the shedding of Giardia parasites by scouring calves, the role of Giardia as a cause of calf diarrhea is still controversial. To elucidate the role of Giardia duodenalis in calf scours, diagnostic samples from 189 scouring calves were tested by different assays during a 1-year-study period. Giardia antigens were detected in 22/189 scouring calves by a fecal-based enzyme-linked immunosorbent assay and 10 of these were positive for assemblage E, G. duodenalis by polymerase chain reaction. Giardia trophozoites were demonstrated by immunohistochemistry in intestinal sections from five calves in which the parasites were spatially distributed in areas of microscopically detectable enteritis. Our data suggest that under certain circumstances, Giardia may cause intestinal lesions leading to calf scours. Gnotobiotic calf-based infectivity studies are needed if the pathogenicity of Giardia in calves is to be definitively determined.
AB - Despite many reports on the shedding of Giardia parasites by scouring calves, the role of Giardia as a cause of calf diarrhea is still controversial. To elucidate the role of Giardia duodenalis in calf scours, diagnostic samples from 189 scouring calves were tested by different assays during a 1-year-study period. Giardia antigens were detected in 22/189 scouring calves by a fecal-based enzyme-linked immunosorbent assay and 10 of these were positive for assemblage E, G. duodenalis by polymerase chain reaction. Giardia trophozoites were demonstrated by immunohistochemistry in intestinal sections from five calves in which the parasites were spatially distributed in areas of microscopically detectable enteritis. Our data suggest that under certain circumstances, Giardia may cause intestinal lesions leading to calf scours. Gnotobiotic calf-based infectivity studies are needed if the pathogenicity of Giardia in calves is to be definitively determined.
KW - Calf scours
KW - Giardia duodenalis
KW - Immunohistochemistry
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U2 - 10.1016/j.vetpar.2008.07.033
DO - 10.1016/j.vetpar.2008.07.033
M3 - Article
C2 - 18774646
AN - SCOPUS:53049093282
SN - 0304-4017
VL - 157
SP - 196
EP - 202
JO - Veterinary Parasitology
JF - Veterinary Parasitology
IS - 3-4
ER -