TY - JOUR
T1 - Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens
AU - Lebdah, Mohamed
AU - Alshaya, Dalal S.
AU - Jalal, Areej S.
AU - Mousa, Mohamed R.
AU - Radwan, Mohamed M.
AU - Samir, Mahmoud
AU - Adel, Amany
AU - Albaqami, Najah M.
AU - El-Saadony, Mohamed T.
AU - El-Tarabily, Khaled A.
AU - El basrey, Yara F.H.
N1 - Funding Information:
The authors extend their appreciation to the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia, for supporting this research work.
Publisher Copyright:
© 2022 The Authors
PY - 2022/12
Y1 - 2022/12
N2 - Inclusion body hepatitis (IBH) is an economically significant viral disease that primarily affects broiler chickens. At least 12 different aviadenovirus serotypes are responsible for causing IBH. This study aimed to use polymerase chain reaction (PCR) and phylogenetic analysis to characterize fowl adenovirus isolates that were in circulation from 2019 to 2021 and investigate the pathogenicity of the isolated strains in commercial broiler chickens. Suspected liver samples were molecularly identified using hexon gene targeting by PCR, and viruses were isolated using chick embryo liver cell culture. For serotype identification, the fowl adenovirus-positive samples were subjected to hexon gene sequencing and phylogenetic analysis. The pathogenicity of two isolates was tested in commercial chickens via the oral route. The phylogenetic analysis of the hexon gene showed that the isolated viruses clustered with serotype 8a species E. On testing the pathogenicity of the isolates based on necropsy and histopathological examination, no mortality was observed; however, lesions were observed in the liver, kidney, heart, pancreas, bursa, and lung specimens with intermittent virus shedding at different time points throughout the experimental period. Further research on the likelihood of vaccine production is warranted to limit disease-related losses.
AB - Inclusion body hepatitis (IBH) is an economically significant viral disease that primarily affects broiler chickens. At least 12 different aviadenovirus serotypes are responsible for causing IBH. This study aimed to use polymerase chain reaction (PCR) and phylogenetic analysis to characterize fowl adenovirus isolates that were in circulation from 2019 to 2021 and investigate the pathogenicity of the isolated strains in commercial broiler chickens. Suspected liver samples were molecularly identified using hexon gene targeting by PCR, and viruses were isolated using chick embryo liver cell culture. For serotype identification, the fowl adenovirus-positive samples were subjected to hexon gene sequencing and phylogenetic analysis. The pathogenicity of two isolates was tested in commercial chickens via the oral route. The phylogenetic analysis of the hexon gene showed that the isolated viruses clustered with serotype 8a species E. On testing the pathogenicity of the isolates based on necropsy and histopathological examination, no mortality was observed; however, lesions were observed in the liver, kidney, heart, pancreas, bursa, and lung specimens with intermittent virus shedding at different time points throughout the experimental period. Further research on the likelihood of vaccine production is warranted to limit disease-related losses.
KW - fowl adenovirus
KW - hexon gene
KW - inclusion body hepatitis
KW - pathogenicity
KW - phylogenetic analysis
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U2 - 10.1016/j.psj.2022.101918
DO - 10.1016/j.psj.2022.101918
M3 - Article
C2 - 36209666
AN - SCOPUS:85139312385
SN - 0032-5791
VL - 101
JO - Poultry Science
JF - Poultry Science
IS - 12
M1 - 101918
ER -