TY - JOUR
T1 - Molecular Characterization of Staphylococcus aureus Isolated from Renal Hemodialysis (HD) Patients from Saudi Arabia
AU - Al-Mogbel, Mohammed S.
AU - Al-Rashid, Fauwaz
AU - Meqdam, Mamdoh
AU - Al-Ajlan, Hisham
AU - Khan, Mushtaq A.
N1 - Funding Information:
This study was funded by King Abdulaziz City for Science and Technology (KACST), General Directorate of Research Grant 143-34.
Publisher Copyright:
© The Author(s) 2019.
PY - 2019/3
Y1 - 2019/3
N2 - Staphylococcus aureus, including methicillin resistant S. aureus (MRSA) is the most commonly isolated pathogen in hospitals worldwide. The aim of present study was molecular characterization of Staphylococcus aureus isolated from renal hemodialysis (HD) patients from Ha’il region of Saudi Arabia. A total of 392 samples were screened from 204 HD patients for colonization of S. aureus. The isolated bacteria were identified by MALDI-TOF-MS. Antibiotic susceptibility testing was performed using Microscan. Among these isolates, 72 S. aureus (43% MRSA and 57% MSSA) were identified.The isolates were considerably resistant with varied profile to the antibiotics tested except being 100% susceptible to vancomycin, linezolid and teicoplanin. Of the isolates, 22.2% were positive for biofilm assay. Four representative MRSA isolates were selected and whole genome sequence analysis was performed using MiSeq. Two out of the 4 MRSA were found to be ST-1 and 2 were found to be ST-32. Among MRSA isolates, 25.8% were negative for mecA and all of them were negative for mecC gene. A high prevalence of MRSA in HD patients as well as high percentage of biofilm production in MRSA isolates highlights the vital role for standardized surveillance along with validated molecular typing methods to evaluate the incidence of MRSA and accordingly to control its spread.
AB - Staphylococcus aureus, including methicillin resistant S. aureus (MRSA) is the most commonly isolated pathogen in hospitals worldwide. The aim of present study was molecular characterization of Staphylococcus aureus isolated from renal hemodialysis (HD) patients from Ha’il region of Saudi Arabia. A total of 392 samples were screened from 204 HD patients for colonization of S. aureus. The isolated bacteria were identified by MALDI-TOF-MS. Antibiotic susceptibility testing was performed using Microscan. Among these isolates, 72 S. aureus (43% MRSA and 57% MSSA) were identified.The isolates were considerably resistant with varied profile to the antibiotics tested except being 100% susceptible to vancomycin, linezolid and teicoplanin. Of the isolates, 22.2% were positive for biofilm assay. Four representative MRSA isolates were selected and whole genome sequence analysis was performed using MiSeq. Two out of the 4 MRSA were found to be ST-1 and 2 were found to be ST-32. Among MRSA isolates, 25.8% were negative for mecA and all of them were negative for mecC gene. A high prevalence of MRSA in HD patients as well as high percentage of biofilm production in MRSA isolates highlights the vital role for standardized surveillance along with validated molecular typing methods to evaluate the incidence of MRSA and accordingly to control its spread.
KW - Hemodialysis
KW - MALDI- TOF-MS
KW - Staphylococcus aureus
KW - Whole genome sequencing Pathogenic bacteria
UR - http://www.scopus.com/inward/record.url?scp=85064002376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064002376&partnerID=8YFLogxK
U2 - 10.22207/JPAM.13.1.28
DO - 10.22207/JPAM.13.1.28
M3 - Article
AN - SCOPUS:85064002376
SN - 0973-7510
VL - 13
SP - 265
EP - 269
JO - Journal of Pure and Applied Microbiology
JF - Journal of Pure and Applied Microbiology
IS - 1
ER -