TY - JOUR
T1 - Molecular characterization of the equine testis-specific protein 1 (TPX1) and acidic epididymal glycoprotein 2 (AEG2) genes encoding members of the cysteine-rich secretory protein (CRISP) family
AU - Giese, Alexander
AU - Jude, Rony
AU - Kuiper, Heidi
AU - Raudsepp, Terje
AU - Piumi, Francois
AU - Schambony, Alexandra
AU - Guérin, Gérard
AU - Chowdhary, Bhanu P.
AU - Distl, Ottmar
AU - Töpfer-Petersen, Edda
AU - Leeb, Tosso
N1 - Funding Information:
The authors would like to thank Heike Klippert and Stefan Neander for expert technical assistance. H.K. and R.J. are supported by the affirmative action program for women from the School of Veterinary Medicine Hannover. This study was supported by grants from the Deutsche Forschungsgemeinschaft DFG (Le1032/6-1 and -2, To114/4-1), the State Ministry for Nutrition, Agriculture, and Forestry of Lower Saxony, and also by Link, TERF and ARP grants 010366-162-2001.
PY - 2002/10/16
Y1 - 2002/10/16
N2 - The cysteine-rich secretory protein (CRISP) family consists of three members called acidic epididymal glycoprotein 1 (AEG1), AEG2, and testis-specific protein 1 (TPX1), which share 16 conserved cysteine residues at their C-termini. The CRISP proteins are primarily expressed in different sections of the male genital tract and are thought to mediate cell-cell interactions of male germ cells with other cells during sperm maturation or during fertilization. Therefore, their genes are of interest as candidate genes for inherited male fertility dysfunctions and as putative quantitative trait loci for male fertility traits. In this report, the cloning and DNA sequence of 137 kb of horse genomic DNA from equine chromosome 20q22 containing the closely linked equine TPX1 and AEG2 genes are described. The equine TPX1 gene consists of ten exons spanning 18 kb while the AEG2 gene consists of eight exons that are spread over 24 kb. The expression of these two genes was investigated in several tissues by reverse transcription polymerase chain reaction analysis and Western blotting. Comparative genome analysis between horse, human, and mouse indicates that all three CRISP genes are clustered on one chromosomal location, which shows conserved synteny between these species.
AB - The cysteine-rich secretory protein (CRISP) family consists of three members called acidic epididymal glycoprotein 1 (AEG1), AEG2, and testis-specific protein 1 (TPX1), which share 16 conserved cysteine residues at their C-termini. The CRISP proteins are primarily expressed in different sections of the male genital tract and are thought to mediate cell-cell interactions of male germ cells with other cells during sperm maturation or during fertilization. Therefore, their genes are of interest as candidate genes for inherited male fertility dysfunctions and as putative quantitative trait loci for male fertility traits. In this report, the cloning and DNA sequence of 137 kb of horse genomic DNA from equine chromosome 20q22 containing the closely linked equine TPX1 and AEG2 genes are described. The equine TPX1 gene consists of ten exons spanning 18 kb while the AEG2 gene consists of eight exons that are spread over 24 kb. The expression of these two genes was investigated in several tissues by reverse transcription polymerase chain reaction analysis and Western blotting. Comparative genome analysis between horse, human, and mouse indicates that all three CRISP genes are clustered on one chromosomal location, which shows conserved synteny between these species.
KW - Acidic epididymal glycoprotein
KW - CRISP-2
KW - CRISP-3
KW - CRISP2
KW - CRISP3
KW - Cysteine-rich secretory protein (CRISP)
KW - Fertility
KW - Horse
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U2 - 10.1016/S0378-1119(02)01018-1
DO - 10.1016/S0378-1119(02)01018-1
M3 - Article
C2 - 12459257
AN - SCOPUS:0037121051
SN - 0378-1119
VL - 299
SP - 101
EP - 109
JO - Gene
JF - Gene
IS - 1-2
ER -