Molecular detection of leptospiral DNA in environmental water on St. Kitts

Julienne Rawlins; Alexandra Portanova; Ilana Zuckerman; Amanda Loftis; Pietro Ceccato; Arve Lee Willingham; Ashutosh Verma

doi:10.3390/ijerph110807953

Molecular detection of leptospiral DNA in environmental water on St. Kitts

Julienne Rawlins, Alexandra Portanova, Ilana Zuckerman, Amanda Loftis, Pietro Ceccato, Arve Lee Willingham, Ashutosh Verma

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA.

Original language	English
Pages (from-to)	7953-7960
Number of pages	8
Journal	International Journal of Environmental Research and Public Health
Volume	11
Issue number	8
DOIs	https://doi.org/10.3390/ijerph110807953
Publication status	Published - Aug 7 2014
Externally published	Yes

Keywords

Environmental transmission
Leptospira
Leptospirosis
Molecular detection

ASJC Scopus subject areas

Pollution
Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/ijerph110807953

Cite this

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title = "Molecular detection of leptospiral DNA in environmental water on St. Kitts",

abstract = "Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA.",

keywords = "Environmental transmission, Leptospira, Leptospirosis, Molecular detection",

author = "Julienne Rawlins and Alexandra Portanova and Ilana Zuckerman and Amanda Loftis and Pietro Ceccato and Willingham, {Arve Lee} and Ashutosh Verma",

year = "2014",

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journal = "International Journal of Environmental Research and Public Health",

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T1 - Molecular detection of leptospiral DNA in environmental water on St. Kitts

AU - Rawlins, Julienne

AU - Portanova, Alexandra

AU - Zuckerman, Ilana

AU - Loftis, Amanda

AU - Ceccato, Pietro

AU - Willingham, Arve Lee

AU - Verma, Ashutosh

PY - 2014/8/7

Y1 - 2014/8/7

N2 - Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA.

AB - Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA.

KW - Environmental transmission

KW - Leptospira

KW - Leptospirosis

KW - Molecular detection

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U2 - 10.3390/ijerph110807953

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Molecular detection of leptospiral DNA in environmental water on St. Kitts

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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