TY - JOUR
T1 - Molecular tools for assuring human health and environment-friendly frozen shellfish products in the United Arab Emirates markets
AU - Galal-Khallaf, Asmaa
AU - Abdelbaset-Donya, Alaa
AU - Hamza, Waleed
AU - Mohammed-Geba, Khaled
N1 - Funding Information:
The authors would like to appreciate their deep thanks to Dr. Mustafa G. Khallaf, Cleveland Dental Institute (USA) for the his aids in the review of the manuscript. Also, we would like to thank the esteemed editor and reviewers of Food Chemistry: Molecular Sciences for their revision and comments that aided us much to enhance the level of the manuscript in general. The manuscript was reviewed for English language by American Language Editors under the project number 83756 (Certificate verification key: 968-114-938-098-346), The study protocol was approved by the Animal Ethics Committee of the Zoology Department, Faculty of Science, Menoufia University, Egypt that bases mainly upon the directive 2010/63/EU of the European Commission regarding the use of animals for experimentation.
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/12/30
Y1 - 2021/12/30
N2 - Shellfish consumption in the United Arab Emirates (UAE) exceeds local supply and frozen fish and seafood products are imported to fill the gap. To determine the species in frozen shellfish brands on the UAE markets, 95 frozen samples were subjected to PCR amplification and sequencing of the hypervariable region of the 16S rDNA. This identified 11 different shrimp species and two squid species in the frozen shellfish packs. About 40% of calamari brands contained peanut worm, cattle, and rat 16S rDNA. Also, most shellfish species analyzed had low nucleotide diversity, including two shrimp species (Litopenaeus vannamei and Metapenopsis barbata), which had very limited genetic diversity, low raggedness, and an absence of population expansion. Species misnaming, substitution, overexploitation, origin misreporting, and low genetic diversity were found across frozen UAE shellfish samples analyzed, suggesting inspection and monitoring of frozen seafood sold in UAE markets would be appropriate.
AB - Shellfish consumption in the United Arab Emirates (UAE) exceeds local supply and frozen fish and seafood products are imported to fill the gap. To determine the species in frozen shellfish brands on the UAE markets, 95 frozen samples were subjected to PCR amplification and sequencing of the hypervariable region of the 16S rDNA. This identified 11 different shrimp species and two squid species in the frozen shellfish packs. About 40% of calamari brands contained peanut worm, cattle, and rat 16S rDNA. Also, most shellfish species analyzed had low nucleotide diversity, including two shrimp species (Litopenaeus vannamei and Metapenopsis barbata), which had very limited genetic diversity, low raggedness, and an absence of population expansion. Species misnaming, substitution, overexploitation, origin misreporting, and low genetic diversity were found across frozen UAE shellfish samples analyzed, suggesting inspection and monitoring of frozen seafood sold in UAE markets would be appropriate.
KW - 16S rDNA
KW - Molecular markers
KW - Seafood markets
KW - Shellfish
KW - UAE
UR - http://www.scopus.com/inward/record.url?scp=85123123153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85123123153&partnerID=8YFLogxK
U2 - 10.1016/j.fochms.2021.100028
DO - 10.1016/j.fochms.2021.100028
M3 - Article
AN - SCOPUS:85123123153
SN - 2666-5662
VL - 3
JO - Food Chemistry: Molecular Sciences
JF - Food Chemistry: Molecular Sciences
M1 - 100028
ER -