TY - JOUR
T1 - Molecular typing and antimicrobial susceptibility of Clostridium perfringens from broiler chickens
AU - Gharaibeh, Saad
AU - Al Rifai, Rami
AU - Al-Majali, Ahmad
N1 - Funding Information:
This project was supported by Deanship of Scientific Research at Jordan University of Science and Technology (Grant # 126/2006 ). We also thank Dr. Saeb El-Sukhon for providing us with the NCTC reference strains.
PY - 2010/12
Y1 - 2010/12
N2 - Clostridium perfringens (Cp) causes necrotic enteritis disease in commercial poultry. Antimicrobials are used to control and treat this disease and sometimes clinical outbreaks do not respond well to certain treatments. This study was designed to isolate Cp from clinical cases, type these isolates by multiplex PCR, and determine their antimicrobial susceptibility by micro-dilution method. A total of 67 Cp isolates were obtained from 155 broiler chicken flocks. All isolates were classified as type A and non-enterotoxin producers. Lincomycin, erythromycins, and tilmicosin showed very high minimal inhibitory concentration (MIC) 50 of ≥256 μg/ml. However, tylosin, amoxicillin, ampicillin, penicillin, florfenicol, danofloxacin, enrofloxacin, chlortetracycline, doxycycline, and oxytetracycline had variable MIC50 of 64, 0.5, 1, 1, 8, 4, 8, 4, 8, 0.5 μg/ml, respectively. It is recommended that Cp infections in Jordan be treated with either penicillins or tetracyclines especially amoxicillin and oxytetracycline.
AB - Clostridium perfringens (Cp) causes necrotic enteritis disease in commercial poultry. Antimicrobials are used to control and treat this disease and sometimes clinical outbreaks do not respond well to certain treatments. This study was designed to isolate Cp from clinical cases, type these isolates by multiplex PCR, and determine their antimicrobial susceptibility by micro-dilution method. A total of 67 Cp isolates were obtained from 155 broiler chicken flocks. All isolates were classified as type A and non-enterotoxin producers. Lincomycin, erythromycins, and tilmicosin showed very high minimal inhibitory concentration (MIC) 50 of ≥256 μg/ml. However, tylosin, amoxicillin, ampicillin, penicillin, florfenicol, danofloxacin, enrofloxacin, chlortetracycline, doxycycline, and oxytetracycline had variable MIC50 of 64, 0.5, 1, 1, 8, 4, 8, 4, 8, 0.5 μg/ml, respectively. It is recommended that Cp infections in Jordan be treated with either penicillins or tetracyclines especially amoxicillin and oxytetracycline.
KW - Chicken
KW - Clostridium perfringens
KW - Minimum inhibitory concentration
KW - Typing
UR - http://www.scopus.com/inward/record.url?scp=78650585330&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650585330&partnerID=8YFLogxK
U2 - 10.1016/j.anaerobe.2010.10.004
DO - 10.1016/j.anaerobe.2010.10.004
M3 - Article
C2 - 20969969
AN - SCOPUS:78650585330
SN - 1075-9964
VL - 16
SP - 586
EP - 589
JO - Anaerobe
JF - Anaerobe
IS - 6
ER -