Multifunctional bioactive properties of hydrolysates from colocynth (Citrullus colocynthis) seeds derived proteins: Characterization and biological properties

Munira Alblooshi, Aarthi Rekha Devarajan, Brij Pal Singh, Preethi Ramakrishnan, Hussein Mostafa, Hina Kamal, Priti Mudgil, Sajid Maqsood

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Citrullus colocynthis (Colocynth) has gained a great deal of interest in their applications as indigenous nutraceutical and as a functional food ingredient. The intact colocynth seed protein was enzymatically hydrolyzed using proteolytic enzymes (alcalase, bromelain, and chymotrypsin) at different time intervals of 3, 6, and 9 h. The highest degree of hydrolysis (87.82%) was observed in chymotrypsin derived colocynth seed protein hydrolysates (CSPH) for 9 h. The CSPHs was further investigated through in-vitro assay to explore its potential biological activity such as antioxidant, inhibition of enzymatic marker related to diabetes (DPP-IV, α-glucosidase and α-amylase) and hyperlipidaemia (cholesteryl esterase and pancreatic lipase). Chymotrypsin hydrolysate showed the strongest DPPH (65.7 mM TEAC) and ABTS (525.2 mM TEAC) radical scavenging activity after 6 h of hydrolysis. Moreover, chymotrypsin-treated CSPH for 6 h inhibited cholesteryl esterase (IC50 = 13.68 μg/mL) and pancreatic lipase (IC50 = 14.12 μg/mL) significantly when compared to native protein. Whereas, bromelain and alcalase treated hydrolysate for 6 h effectively inhibited α-glucosidase and α-amylase at an inhibitory concentration of IC50 = 13.27 μg/mL and of IC50 = 17 μg/mL. Overall, the findings indicated that protein hydrolysates exhibited superior biological activity than intact colocynth seed proteins isolate (CSPI) and could be a sustainable source of bioactive peptides.

Original languageEnglish
Pages (from-to)326-334
Number of pages9
JournalPlant Physiology and Biochemistry
Volume194
DOIs
Publication statusPublished - Jan 2023

Keywords

  • Anti-diabetic
  • Anti-hyperlipidaemia
  • Antioxidant
  • Colocynth
  • Protein hydrolysates

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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