Muscarinic M3 receptor subtype gene expression in the human heart

I. Hellgren; A. Mustafa; M. Riazi; I. Suliman; C. Sylvén; A. Adem

doi:10.1007/s000180050507

Muscarinic M₃ receptor subtype gene expression in the human heart

I. Hellgren, A. Mustafa, M. Riazi, I. Suliman, C. Sylvén, A. Adem

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

The heart is an important target organ for cholinergic function. In this study, muscarinic receptor subtype(s) in the human heart were determined using reverse transcription-polymerase chain reaction. Our results demonstrated muscarinic receptor M₂ and M₃ subtype RNA in left/right atria/ventricles of donor hearts. Receptor autoradiography analysis using selective muscarinic ligands indicated an absence of M₁ receptor subtype in the human heart. The level of muscarinic receptor binding in atria was two to three times greater than in ventricles. Our results suggest that muscarinic receptors in the human heart are of the M₂ and M₃ subtypes. This is the first report of M₃ receptors in the human myocardium.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Cellular and Molecular Life Sciences
Volume	57
Issue number	1
DOIs	https://doi.org/10.1007/s000180050507
Publication status	Published - 2000
Externally published	Yes

Keywords

Atrium
Heart failure
Human
Muscarinic receptor
Protein
Subtypes
Ventricle
mRNA

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Pharmacology
Cellular and Molecular Neuroscience
Cell Biology

Access to Document

10.1007/s000180050507

Cite this

@article{a91a07067a384b35bf3d022725923c1c,

title = "Muscarinic M3 receptor subtype gene expression in the human heart",

abstract = "The heart is an important target organ for cholinergic function. In this study, muscarinic receptor subtype(s) in the human heart were determined using reverse transcription-polymerase chain reaction. Our results demonstrated muscarinic receptor M2 and M3 subtype RNA in left/right atria/ventricles of donor hearts. Receptor autoradiography analysis using selective muscarinic ligands indicated an absence of M1 receptor subtype in the human heart. The level of muscarinic receptor binding in atria was two to three times greater than in ventricles. Our results suggest that muscarinic receptors in the human heart are of the M2 and M3 subtypes. This is the first report of M3 receptors in the human myocardium.",

keywords = "Atrium, Heart failure, Human, Muscarinic receptor, Protein, Subtypes, Ventricle, mRNA",

author = "I. Hellgren and A. Mustafa and M. Riazi and I. Suliman and C. Sylv{\'e}n and A. Adem",

year = "2000",

doi = "10.1007/s000180050507",

language = "English",

volume = "57",

pages = "175--180",

journal = "Cellular and Molecular Life Sciences",

issn = "1420-682X",

publisher = "Birkhauser Verlag Basel",

number = "1",

}

TY - JOUR

T1 - Muscarinic M3 receptor subtype gene expression in the human heart

AU - Hellgren, I.

AU - Mustafa, A.

AU - Riazi, M.

AU - Suliman, I.

AU - Sylvén, C.

AU - Adem, A.

PY - 2000

Y1 - 2000

N2 - The heart is an important target organ for cholinergic function. In this study, muscarinic receptor subtype(s) in the human heart were determined using reverse transcription-polymerase chain reaction. Our results demonstrated muscarinic receptor M2 and M3 subtype RNA in left/right atria/ventricles of donor hearts. Receptor autoradiography analysis using selective muscarinic ligands indicated an absence of M1 receptor subtype in the human heart. The level of muscarinic receptor binding in atria was two to three times greater than in ventricles. Our results suggest that muscarinic receptors in the human heart are of the M2 and M3 subtypes. This is the first report of M3 receptors in the human myocardium.

AB - The heart is an important target organ for cholinergic function. In this study, muscarinic receptor subtype(s) in the human heart were determined using reverse transcription-polymerase chain reaction. Our results demonstrated muscarinic receptor M2 and M3 subtype RNA in left/right atria/ventricles of donor hearts. Receptor autoradiography analysis using selective muscarinic ligands indicated an absence of M1 receptor subtype in the human heart. The level of muscarinic receptor binding in atria was two to three times greater than in ventricles. Our results suggest that muscarinic receptors in the human heart are of the M2 and M3 subtypes. This is the first report of M3 receptors in the human myocardium.

KW - Atrium

KW - Heart failure

KW - Human

KW - Muscarinic receptor

KW - Protein

KW - Subtypes

KW - Ventricle

KW - mRNA

UR - http://www.scopus.com/inward/record.url?scp=0034688053&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034688053&partnerID=8YFLogxK

U2 - 10.1007/s000180050507

DO - 10.1007/s000180050507

M3 - Article

C2 - 10949589

AN - SCOPUS:0034688053

SN - 1420-682X

VL - 57

SP - 175

EP - 180

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

IS - 1

ER -