Mutated TATA-box/TATA binding protein complementation system for regulated transgene expression in tobacco

Chandra Prakash Chaturvedi, Niraj Lodhi, Suraiya A. Ansari, Siddharth Tiwari, Rakesh Srivastava, Samir V. Sawant, Rakesh Tuli

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

A two-component expression system was developed to achieve tightly regulated expression of transgenes in plants. One component functioned as an expression module whereas the other functioned as a regulatory module. The expression module comprised a highly expressing TATA-dependent seed-specific promoter in which the TATA motif in the core promoter was mutated to TGTA. The regulatory module expressed a mutated general transcription factor TBPm 3 that recognized TGTA and initiated transcription. Vectors were designed using component one alone or in combination with component two, and were transformed into tobacco. The TGTA mutation in the TATA-box completely inactivated the promoter, making component one non-functional. This non-functional module became transcriptionally active in the presence of the component two that expressed TBPm3. The reporter gene gusA was expressed from the TGTA-containing chimeric legumin promoter, in a tightly seed-specific manner, in transgenic tobacco plants in the presence of TBPm 3 that was expressed from a constitutive promoter. The results show that the TGTA and TBPm3 combination can be used to achieve high-level tissue-specific expression of TATA-dependent promoters.

Original languageEnglish
Pages (from-to)917-925
Number of pages9
JournalPlant Journal
Volume50
Issue number5
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Keywords

  • Expression module
  • Legumin promoter
  • TATA binding protein
  • TATA box
  • TBP mutant
  • Tightly regulated promoter

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint

Dive into the research topics of 'Mutated TATA-box/TATA binding protein complementation system for regulated transgene expression in tobacco'. Together they form a unique fingerprint.

Cite this