Abstract
A two-component expression system was developed to achieve tightly regulated expression of transgenes in plants. One component functioned as an expression module whereas the other functioned as a regulatory module. The expression module comprised a highly expressing TATA-dependent seed-specific promoter in which the TATA motif in the core promoter was mutated to TGTA. The regulatory module expressed a mutated general transcription factor TBPm 3 that recognized TGTA and initiated transcription. Vectors were designed using component one alone or in combination with component two, and were transformed into tobacco. The TGTA mutation in the TATA-box completely inactivated the promoter, making component one non-functional. This non-functional module became transcriptionally active in the presence of the component two that expressed TBPm3. The reporter gene gusA was expressed from the TGTA-containing chimeric legumin promoter, in a tightly seed-specific manner, in transgenic tobacco plants in the presence of TBPm 3 that was expressed from a constitutive promoter. The results show that the TGTA and TBPm3 combination can be used to achieve high-level tissue-specific expression of TATA-dependent promoters.
Original language | English |
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Pages (from-to) | 917-925 |
Number of pages | 9 |
Journal | Plant Journal |
Volume | 50 |
Issue number | 5 |
DOIs | |
Publication status | Published - Jun 2007 |
Externally published | Yes |
Keywords
- Expression module
- Legumin promoter
- TATA binding protein
- TATA box
- TBP mutant
- Tightly regulated promoter
ASJC Scopus subject areas
- Genetics
- Plant Science
- Cell Biology