@article{46a2f40076e3444aa0a6dce0dc307620,
title = "Nano-evolution and protein-based enzymatic evolution predicts novel types of natural product nanozymes of traditional Chinese medicine: cases of herbzymes of Taishan-Huangjing (Rhizoma polygonati) and Goji (Lycium chinense)",
abstract = "Nanozymes and natural product-derived herbzymes have been identified in different types of enzymes simulating the natural protein-based enzyme function. How to explore and predict enzyme types of novel nanozymes when synthesized remains elusive. An informed analysis might be useful for the prediction. Here, we applied a protein-evolution analysis method to predict novel types of enzymes with experimental validation. First, reported nanozymes were analyzed by chemical classification and nano-evolution. We found that nanozymes are predominantly classified as protein-based EC1 oxidoreductase. In comparison, we analyzed the evolution of protein-based natural enzymes by a phylogenetic tree and the most conserved enzymes were found to be peroxidase and lyase. Therefore, the natural products ofRhizoma polygonatiand Goji herbs were analyzed to explore and test the potent new types of natural nanozymes/herbzymes using the simplicity simulation of natural protein enzyme evolution as they contain these conserved enzyme types. The experimental validation showed that the natural products from the total extract of nanoscale traditional Chinese medicine Huangjing (RP,Rhizoma polygonati) from Mount-Tai (Taishan) exhibit fructose-bisphosphate aldolase of lyase while nanoscale Goji (Lycium chinense) extract exhibits peroxidase activities. Thus, the bioinformatics analysis would provide an additional tool for the virtual discovery of natural product nanozymes.",
author = "Guldan Nazarbek and Aidana Kutzhanova and Lazzat Nurtay and Chenglin Mu and Bexultan Kazybay and Xugang Li and Cuiping Ma and Amr Amin and Yingqiu Xie",
note = "Funding Information: We would like to thank the undergraduate students such as Assel Ibadulla who contributed to their support, assistance and valuable comments. The authors thank the Nazarbayev University Faculty-Development Competitive Research Grants Program (ID of 16797152 and ID 16796808), Shandong Taishanghuangjing Biotechnology Co. Ltd., Taian, and UAEU 2019 AUA fellowship. Funding Information: We would like to thank the undergraduate students such as Assel Ibadulla who contributed to their support, assistance and valuable comments. The authors thank the Nazarbayev University Faculty-Development Competitive Research Grants Program (ID of 16797152 and ID 16796808), Shandong Taish-anghuangjing Biotechnology Co. Ltd., Taian, and UAEU 2019 AUA fellowship. Funding Information: Yingqiu Xie received the research grant from the Nazarbayev University Faculty-Development Competitive Research Grants Program (ID 16797152) with title of “Targeting cancer stem-like cells of castration-resistant prostate cancer through combinatorial inhibition of MET/nuclear MET and b-Catenin pathways: potential therapeutic intellectual property in prostate cancer treatment (110119FD4531)” to YX and (ID 16796808) with title of “Phosphatase-like nanozyme activity of carbon nanodots and its potential as supplement for kinase inhibitor drug treating prostate cancer: potential intellectual property discovered in food product (110119FD4542)” to HF and YX. YX thanks the United Arab Emirates University (UAEU) – 2019 Asian Universities Alliance (AUA) fellowship (YX and AA). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",
year = "2021",
month = dec,
day = "7",
doi = "10.1039/d1na00475a",
language = "English",
volume = "3",
pages = "6728--6738",
journal = "Nanoscale Advances",
issn = "2516-0230",
publisher = "Royal Society of Chemistry",
number = "23",
}