TY - JOUR
T1 - Neuropeptides in the rat corpus cavernosum and seminal vesicle
T2 - Effects of age and two types of diabetes
AU - Morrison, J. F.B.
AU - Dhanasekaran, S.
AU - Howarth, F. C.
N1 - Funding Information:
We would like to acknowledge the support of the Faculty of Medicine and Health Sciences, UAEU Research Affairs and the Sheikh Hamdan Award for Medical Sciences.
PY - 2009/3/12
Y1 - 2009/3/12
N2 - Aims: To observe the development of neuropathic changes in two types of experimental diabetes using changes in concentrations of NPY, CGRP and amines in the corpora cavernosa and seminal vesicles. Type I diabetes was studied in Wistar rats after 12 and 16 weeks of STZ-induced hyperglycaemia, and Type II diabetes was studied in prediabetic GK rats aged 52 weeks. Both were compared with age-matched normal Wistar rats. Methods: NPY and CGRP were estimated using radioimmunoassay, and amines using HPLC. Results: There were significant changes in [CGRP] in the normal corpus cavernosum and in [NPY] in the normal seminal vesicle with age. STZ-diabetes, induced at 10 weeks of age, resulted in significant elevation of [NPY] and [CGRP] in the corpora cavernosa and seminal vesicles after 12 and 16 weeks of hyperglycaemia, relative to age-matched control rats. The GK rats were intolerant of glucose at 52 weeks of age, but did not have raised fasting blood glucose levels. [NPY], [CGRP] and [noradrenaline] in corpora cavernosa were significantly increased in the prediabetic GK animals relative to age-matched Wistar control rats. The seminal vesicles of GK rats showed a significant increase in [NPY], a non-significant increase in [CGRP], and a fall in [noradrenaline] relative to the age-matched Wistar controls. Conclusions: The results indicate increased levels of NPY and noradrenaline in autonomic nerves, and of CGRP in sensory nerves, innervating the corpus cavernosum in Type I and in prediabetic Type II GK rats.
AB - Aims: To observe the development of neuropathic changes in two types of experimental diabetes using changes in concentrations of NPY, CGRP and amines in the corpora cavernosa and seminal vesicles. Type I diabetes was studied in Wistar rats after 12 and 16 weeks of STZ-induced hyperglycaemia, and Type II diabetes was studied in prediabetic GK rats aged 52 weeks. Both were compared with age-matched normal Wistar rats. Methods: NPY and CGRP were estimated using radioimmunoassay, and amines using HPLC. Results: There were significant changes in [CGRP] in the normal corpus cavernosum and in [NPY] in the normal seminal vesicle with age. STZ-diabetes, induced at 10 weeks of age, resulted in significant elevation of [NPY] and [CGRP] in the corpora cavernosa and seminal vesicles after 12 and 16 weeks of hyperglycaemia, relative to age-matched control rats. The GK rats were intolerant of glucose at 52 weeks of age, but did not have raised fasting blood glucose levels. [NPY], [CGRP] and [noradrenaline] in corpora cavernosa were significantly increased in the prediabetic GK animals relative to age-matched Wistar control rats. The seminal vesicles of GK rats showed a significant increase in [NPY], a non-significant increase in [CGRP], and a fall in [noradrenaline] relative to the age-matched Wistar controls. Conclusions: The results indicate increased levels of NPY and noradrenaline in autonomic nerves, and of CGRP in sensory nerves, innervating the corpus cavernosum in Type I and in prediabetic Type II GK rats.
KW - Aging
KW - Autonomic nervous system
KW - Corpus Cavernosum
KW - Diabetes
KW - Neuropathy
KW - Peptides
KW - Sympathetic nervous system
UR - http://www.scopus.com/inward/record.url?scp=60349118601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60349118601&partnerID=8YFLogxK
U2 - 10.1016/j.autneu.2008.11.016
DO - 10.1016/j.autneu.2008.11.016
M3 - Article
C2 - 19152794
AN - SCOPUS:60349118601
SN - 1566-0702
VL - 146
SP - 76
EP - 80
JO - Autonomic Neuroscience: Basic and Clinical
JF - Autonomic Neuroscience: Basic and Clinical
IS - 1-2
ER -