New embedding medium for sectioning undecalcified bone

S. Mohsin, F. J. O'Brien, T. C. Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Methylmethacrylate (MMA) is the most commonly used embedding medium for sectioning undecalcified bone; however, a number of problems exist with its use in a research laboratory. MMA requires a long infiltration time and temperature control, and it reacts with many polymers. We used Kleer Set resin™ as an alternative embedding medium for sectioning undecalcified bone specimens. Fluorochrome labeled bone specimens were sectioned transversely using a ground section technique and longitudinally on a sledge macrotome. The slides were viewed using both transmitted light and epifluorescence microscopy. High quality sections were obtained using Kleer Set resin™ for both sectioning techniques. We have shown that this new embedding medium is simpler, safer, quicker to use and does not interfere with visualization of fluorochromes.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalBiotechnic and Histochemistry
Issue number2-3
Publication statusPublished - Mar 2006
Externally publishedYes


  • Bone
  • Embedding
  • Epifluorescence microscopy
  • Media
  • Resin
  • Sectioning

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology


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