No impact of strongylid infections on the detection of Plasmodium spp. in faeces of western lowland gorillas and eastern chimpanzees

Mwanahamisi I. Mapua; Barbora Pafčo; Jade Burgunder; Ilona Profousová-Pšenková; Angelique Todd; Chie Hashimoto; Moneeb A. Qablan; David Modrý; Klára J. Petrželková

doi:10.1186/s12936-017-1822-z

No impact of strongylid infections on the detection of Plasmodium spp. in faeces of western lowland gorillas and eastern chimpanzees

Mwanahamisi I. Mapua, Barbora Pafčo, Jade Burgunder, Ilona Profousová-Pšenková, Angelique Todd, Chie Hashimoto, Moneeb A. Qablan, David Modrý, Klára J. Petrželková

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Background: Although a high genetic diversity of Plasmodium spp. circulating in great apes has been revealed recently due to non-invasive methods enabling detection in faecal samples, little is known about the actual mechanisms underlying the presence of Plasmodium DNA in faeces. Great apes are commonly infected by strongylid nematodes, including hookworms, which cause intestinal bleeding. The impact of strongylid infections on the detection of Plasmodium DNA in faeces was assessed in wild, western, lowland gorillas from Dzanga Sangha Protected Areas, Central African Republic and eastern chimpanzees from Kalinzu Forest Reserve, Uganda. Methods: Fifty-one faecal samples from 22 habituated gorillas and 74 samples from 15 habituated chimpanzees were analysed using Cytochrome-b PCR assay and coprological methods. Results: Overall, 26.4% of the analysed samples were positive for both Plasmodium spp. and strongylids. However, the results showed no significant impact of intensity of infections of strongylids on detection of Plasmodium DNA in gorilla and chimpanzee faeces. Conclusion: Bleeding caused by strongylid nematode Necator spp. cannot explain the presence of Plasmodium DNA in ape faeces.

Original language	English
Article number	175
Journal	Malaria Journal
Volume	16
Issue number	1
DOIs	https://doi.org/10.1186/s12936-017-1822-z
Publication status	Published - Apr 26 2017
Externally published	Yes

Keywords

Co-infection
Eastern chimpanzee
Faeces
Malaria
Necator spp.
Plasmodium spp.
Strongylid
Western lowland gorilla

ASJC Scopus subject areas

Parasitology
Infectious Diseases

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10.1186/s12936-017-1822-z

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@article{e4558390c07346c49320232236936a5f,

title = "No impact of strongylid infections on the detection of Plasmodium spp. in faeces of western lowland gorillas and eastern chimpanzees",

abstract = "Background: Although a high genetic diversity of Plasmodium spp. circulating in great apes has been revealed recently due to non-invasive methods enabling detection in faecal samples, little is known about the actual mechanisms underlying the presence of Plasmodium DNA in faeces. Great apes are commonly infected by strongylid nematodes, including hookworms, which cause intestinal bleeding. The impact of strongylid infections on the detection of Plasmodium DNA in faeces was assessed in wild, western, lowland gorillas from Dzanga Sangha Protected Areas, Central African Republic and eastern chimpanzees from Kalinzu Forest Reserve, Uganda. Methods: Fifty-one faecal samples from 22 habituated gorillas and 74 samples from 15 habituated chimpanzees were analysed using Cytochrome-b PCR assay and coprological methods. Results: Overall, 26.4% of the analysed samples were positive for both Plasmodium spp. and strongylids. However, the results showed no significant impact of intensity of infections of strongylids on detection of Plasmodium DNA in gorilla and chimpanzee faeces. Conclusion: Bleeding caused by strongylid nematode Necator spp. cannot explain the presence of Plasmodium DNA in ape faeces.",

keywords = "Co-infection, Eastern chimpanzee, Faeces, Malaria, Necator spp., Plasmodium spp., Strongylid, Western lowland gorilla",

author = "Mapua, {Mwanahamisi I.} and Barbora Paf{\v c}o and Jade Burgunder and Ilona Profousov{\'a}-P{\v s}enkov{\'a} and Angelique Todd and Chie Hashimoto and Qablan, {Moneeb A.} and David Modr{\'y} and Petr{\v z}elkov{\'a}, {Kl{\'a}ra J.}",

note = "Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = apr,

day = "26",

doi = "10.1186/s12936-017-1822-z",

language = "English",

volume = "16",

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T1 - No impact of strongylid infections on the detection of Plasmodium spp. in faeces of western lowland gorillas and eastern chimpanzees

AU - Mapua, Mwanahamisi I.

AU - Pafčo, Barbora

AU - Burgunder, Jade

AU - Profousová-Pšenková, Ilona

AU - Todd, Angelique

AU - Hashimoto, Chie

AU - Qablan, Moneeb A.

AU - Modrý, David

AU - Petrželková, Klára J.

PY - 2017/4/26

Y1 - 2017/4/26

N2 - Background: Although a high genetic diversity of Plasmodium spp. circulating in great apes has been revealed recently due to non-invasive methods enabling detection in faecal samples, little is known about the actual mechanisms underlying the presence of Plasmodium DNA in faeces. Great apes are commonly infected by strongylid nematodes, including hookworms, which cause intestinal bleeding. The impact of strongylid infections on the detection of Plasmodium DNA in faeces was assessed in wild, western, lowland gorillas from Dzanga Sangha Protected Areas, Central African Republic and eastern chimpanzees from Kalinzu Forest Reserve, Uganda. Methods: Fifty-one faecal samples from 22 habituated gorillas and 74 samples from 15 habituated chimpanzees were analysed using Cytochrome-b PCR assay and coprological methods. Results: Overall, 26.4% of the analysed samples were positive for both Plasmodium spp. and strongylids. However, the results showed no significant impact of intensity of infections of strongylids on detection of Plasmodium DNA in gorilla and chimpanzee faeces. Conclusion: Bleeding caused by strongylid nematode Necator spp. cannot explain the presence of Plasmodium DNA in ape faeces.

AB - Background: Although a high genetic diversity of Plasmodium spp. circulating in great apes has been revealed recently due to non-invasive methods enabling detection in faecal samples, little is known about the actual mechanisms underlying the presence of Plasmodium DNA in faeces. Great apes are commonly infected by strongylid nematodes, including hookworms, which cause intestinal bleeding. The impact of strongylid infections on the detection of Plasmodium DNA in faeces was assessed in wild, western, lowland gorillas from Dzanga Sangha Protected Areas, Central African Republic and eastern chimpanzees from Kalinzu Forest Reserve, Uganda. Methods: Fifty-one faecal samples from 22 habituated gorillas and 74 samples from 15 habituated chimpanzees were analysed using Cytochrome-b PCR assay and coprological methods. Results: Overall, 26.4% of the analysed samples were positive for both Plasmodium spp. and strongylids. However, the results showed no significant impact of intensity of infections of strongylids on detection of Plasmodium DNA in gorilla and chimpanzee faeces. Conclusion: Bleeding caused by strongylid nematode Necator spp. cannot explain the presence of Plasmodium DNA in ape faeces.

KW - Co-infection

KW - Eastern chimpanzee

KW - Faeces

KW - Malaria

KW - Necator spp.

KW - Plasmodium spp.

KW - Strongylid

KW - Western lowland gorilla

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SN - 1475-2875

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ER -

No impact of strongylid infections on the detection of Plasmodium spp. in faeces of western lowland gorillas and eastern chimpanzees

Abstract

Keywords

ASJC Scopus subject areas

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